UNSPECIFIED (1995) THE GLYCOSYLCERAMIDES OF THE NEMATODE CAENORHABDITIS-ELEGANS CONTAIN AN UNUSUAL, BRANCHED-CHAIN SPHINGOID BASE. LIPIDS, 30 (6). pp. 567-573. ISSN 0024-4201

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Abstract

Caenorhabditis elegans was cultured in semi-defined medium containing yeast extract, soy peptone, glucose, hemoglobin, Tween 80, and sitosterol. Monoglycosylceramides were chromatographically purified from nematode extracts. Their structures were elucidated with mass spectrometry, nuclear magnetic resonance spectroscopy, and analysis of methanolysis products of the parent cerebrosides. The glycosylceramides were unusual in that the only long-chain sphingoid base detected was an iso-branched compound with a C-4 double bond (i.e., 15-methyl-2-aminohexadec-4-en-1,3-diol). Glucose was the only sugar moiety detected. The fatty acids consisted of a series of primarily straight-chain, saturated, 2-hydroxylated C-20-C-26 acids; some iso-branched analogs also occurred. The sphingomyelins of C. elegans were also hydrolyzed, and the same iso-branched C-17 compound was the only sphingoid base detected. This is the first structural analysis of a nematode glycosphingolipid and the first report of an organism in which the long-chain sphingoid bases are entirely iso-branched.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry R Medicine > RA Public aspects of medicine
Journal or Publication Title:	LIPIDS
Publisher:	AMER OIL CHEMISTS SOC
ISSN:	0024-4201
Date:	June 1995
Volume:	30
Number:	6
Number of Pages:	7
Page Range:	pp. 567-573
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/19759

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