Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

THE EFFECTS OF SURFACTANTS ON LIPASE-CATALYZED HYDROLYSIS OF ESTERS - ACTIVITIES AND STEREOSELECTIVITY

Tools
- Tools
+ Tools

UNSPECIFIED (1994) THE EFFECTS OF SURFACTANTS ON LIPASE-CATALYZED HYDROLYSIS OF ESTERS - ACTIVITIES AND STEREOSELECTIVITY. BIOCATALYSIS, 11 (3). pp. 191-221.

Research output not available from this repository, contact author.

Request Changes to record.

Abstract

The effect of surfactants on the hydrolysis of prochiral and chiral substrates by crude and purified porcine pancreatic lipase (PPL, EC 3.1.1.3)) has been studied. Rather than accelerating the reactions, surfactants slowed down (''inhibited'') the reactions relative to the rate in the absence of surfactant. Surfactants varied in the extent to which the reaction was inhibited. With the crude enzyme there was a correlation between degree of inhibition and the optical purity of the product of hydrolysis of an achiral diester substrate 1. There was no special effect associated with use of surfactants in the concentration range corresponding to critical micelle formation, nor was there any increase in rate of reaction when stable emulsions were formed by using mixtures of surfactants to generate an appropriate hydrophile-lipophile (HLB) balance. A study of the effect of sodium dodecyl sulphate (SDS) on the hydrolysis of the diester 1 by crude PPL showed that the rate of the reaction steadily decreased with increasing surfactant concentration, but that the optical purity of the product first fell and then rose gain, an effect attributed to the differential denaturing action of the surfactant on at least three hydrolytic enzymes. In general, there would seem to be no advantage to be gained from the use of surfactants in the hydrolysis by PPL of compounds of low water solubility; the use of an immiscible co-solvent is more effective.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
T Technology > TP Chemical technology
Journal or Publication Title: BIOCATALYSIS
Publisher: HARWOOD ACAD PUBL GMBH
ISSN: 0886-4454
Official Date: 1994
Dates:
DateEvent
1994UNSPECIFIED
Volume: 11
Number: 3
Number of Pages: 31
Page Range: pp. 191-221
Publication Status: Published

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us