UNSPECIFIED. (1995) EXPRESSION OF ADENOVIRUS TYPE-5 E4 ORF2 PROTEIN DURING LYTIC INFECTION. JOURNAL OF GENERAL VIROLOGY, 76 (Part 4). pp. 1051-1055. ISSN 0022-1317

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Abstract

The human adenovirus type 5 E4 transcription unit has the potential to encode at least seven distinct polypeptides from reading frames accessed by differential splicing of a single primary transcript. Only some of these polypeptides have yet been detected during viral infection of cultured cells. Mutational inactivation of the reading frames whose products have not been described has no apparent effect on the growth of virus in standard cultured human cell lines, indicating that these proteins, if they exist, have only a subtle, non-essential role in the replication cycle. We have raised an antiserum to one of these undefined products, E4 Orf2, expressed in bacteria. Using this reagent, it was possible to show that Orf2 was expressed during the lytic cycle in HeLa cells, being a soluble cytoplasmic component appearing with early kinetics. No association of Orf2 protein with other infected cell components was detected.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title:	JOURNAL OF GENERAL VIROLOGY
Publisher:	SOC GENERAL MICROBIOLOGY
ISSN:	0022-1317
Official Date:	April 1995
Dates:	Date Event April 1995 UNSPECIFIED
Volume:	76
Number:	Part 4
Number of Pages:	5
Page Range:	pp. 1051-1055
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/19907

Data sourced from Thomson Reuters' Web of Knowledge

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