UNSPECIFIED (1995) METHANE MONOOXYGENASE MUTANTS OF METHYLOSINUS-TRICHOSPORIUM CONSTRUCTED BY MARKER-EXCHANGE MUTAGENESIS. FEMS MICROBIOLOGY LETTERS, 127 (3). pp. 243-248. ISSN 0378-1097

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Abstract

Methylosinus trichosporium OB3b synthesizes a soluble cytoplasmic methane monooxygenase when grown in copper-depleted medium and a membrane-bound particulate methane monooxygenase under copper-replete conditions. The genes encoding the hydroxylase component of soluble methane monooxygenase, carried on a plasmid in Escherichia coli, were insertionally inactivated using a kanamycin cassette and transferred back into M. trichosporium by conjugation. Marker-exchange mutagenesis, via a double homologous recombination event, yielded a soluble methane monooxygenase-negative mutant which grew only on methane using the particulate methane monooxygenase during copper-replete growth conditions, thus proving that the two methane oxidation systems in this methanotroph are genetically distinct.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	FEMS MICROBIOLOGY LETTERS
Publisher:	ELSEVIER SCIENCE BV
ISSN:	0378-1097
Date:	1 April 1995
Volume:	127
Number:	3
Number of Pages:	6
Page Range:	pp. 243-248
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/19929

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