DETECTION OF METHANOTROPHIC BACTERIA IN ENVIRONMENTAL-SAMPLES WITH THE PCR
UNSPECIFIED. (1995) DETECTION OF METHANOTROPHIC BACTERIA IN ENVIRONMENTAL-SAMPLES WITH THE PCR. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 61 (1). pp. 116-121. ISSN 0099-2240Full text not available from this repository.
We designed PCR primers by using the DNA sequences of the soluble methane monooxygenase gene clusters of Methylosinus trichosporium OB3b and Methylococcus capsulatus (Bath), and these primers were found to be specific for four of the five structural genes in the soluble methane monooxygenase gene clusters of several methanotrophs. We also designed primers for the gram-negative methylotroph-specific methanol dehydrogenase gene moxF. The specificity of these primers was confirmed by hybridizing and sequencing the PCR products obtained. The primers were then used to amplify methanotroph DNAs in samples obtained from various aquatic and terrestrial environments. Our sequencing data suggest that a large number of different methanotrophs are present in peat samples and also that there is a high level of variability in the mmoC gene, which codes for the reductase component of the soluble methane monooxygenase, while the mmoX gene, which codes for the alpha subunit of the hydroxylase component of this enzyme complex, appears to be highly conserved in methanotrophs.
|Item Type:||Journal Article|
|Subjects:||T Technology > TP Chemical technology
Q Science > QR Microbiology
|Journal or Publication Title:||APPLIED AND ENVIRONMENTAL MICROBIOLOGY|
|Publisher:||AMER SOC MICROBIOLOGY|
|Official Date:||January 1995|
|Number of Pages:||6|
|Page Range:||pp. 116-121|
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