Identification and sequence analysis of the soxB gene essential for sulfur oxidation of Paracoccus denitrificans GB17
Wodara, C., Kostka, S., Egert, M., Kelly, Donovan P. and Friedrich, C.G. (1994) Identification and sequence analysis of the soxB gene essential for sulfur oxidation of Paracoccus denitrificans GB17. Journal of Bacteriology, Vol.176 (No.20). pp. 6188-6191. ISSN 0021-9193Full text not available from this repository.
Official URL: http://jb.asm.org/content/176/20/6188.abstract
The coding region for lithotrophic sulfur oxidation (Sox) in Paracoccus denitrificans GB17 was identified by isolation of a transposon Tn5-mob mutant with a Sox(-) phenotype (strain TP19). The corresponding wild-type region was cloned previously (G. Mittenhuber, K. Sonomoto, M. Egert, and C. G. Friedrich, J. Bacteriol. 173:7340-7344, 1991). Sequence analysis of a 2.5-kb subclone that complemented strain TP19 revealed that Tn5-mob was inserted into a coding region for a 553-amino-acid polypeptide named SoxB. This polypeptide had an M(r) of 60.573, including a possible signal peptide. The function of the SoxB protein of P. denitrificans GB17 appeared to be identical to that of enzyme B of the thiosulfate-oxidizing enzyme system of Thiobacillus versutus. The amino acid compositions of the two proteins mere identical, and the amino acid sequences of three internal peptides of enzyme B as determined by Edman degradation were identical to corresponding sequences of the deduced SoxB protein of P. denitrificans GB17.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology|
|Divisions:||Faculty of Science > Life Sciences (2010- )|
|Journal or Publication Title:||Journal of Bacteriology|
|Publisher:||American Society for Microbiology|
|Official Date:||October 1994|
|Number of Pages:||4|
|Page Range:||pp. 6188-6191|
|Access rights to Published version:||Restricted or Subscription Access|
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