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TUMOR-NECROSIS-FACTOR-ALPHA AND INTERLEUKIN-1-BETA INDUCE SPECIFIC SUBUNITS OF NF-KAPPAB TO BIND THE HIV-1 ENHANCER - CHARACTERIZATION OF TRANSCRIPTION FACTORS CONTROLLING HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GENE-EXPRESSION IN NEURAL CELLS
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UNSPECIFIED (1994) TUMOR-NECROSIS-FACTOR-ALPHA AND INTERLEUKIN-1-BETA INDUCE SPECIFIC SUBUNITS OF NF-KAPPAB TO BIND THE HIV-1 ENHANCER - CHARACTERIZATION OF TRANSCRIPTION FACTORS CONTROLLING HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GENE-EXPRESSION IN NEURAL CELLS. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 203 (1). pp. 623-630.
Research output not available from this repository, contact author.Abstract
In human astrocytoma and neuroblastoma cell lines tumour necrosis factor alpha (TNF alpha) and interleukin 1 beta (IL-1 beta) induced NFKB and an additional KB-specific protein of approximately 80 K to bind the HIV-1 enhancer. One nucleoprotein complex contained prototypical NFKB comprising of p50 and p65 subunits and a second contained the p65 subunit and an 80 K factor, p80. Over a 24 hr period of cytokine stimulation the p50/p65 complex of NFKB was present in the nucleus whilst the second complex declined in abundance after two hours due to the decline of p80. In unstimulated cells, DNAse I footprinting revealed a previously unidentified octamer-like binding site in the negative regulatory element (NRE) of the HIV-1 long terminal repeat (LTR) which specifically bound protein factors present in human astrocytoma, neuroblastoma and murine oligodendroglioma cell lines. A second unique motif, also in the NRE, was observed with extracts from one human neuroblastoma cell line and a murine oligodendroglioma. Footprinting analysis also confirmed that Sp1, TATA, Site A and Site B motifs of the LTR were occupied by nuclear factors present in neural cells (C) 1994 Academic Press, Inc.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
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| Journal or Publication Title: | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | ||||
| Publisher: | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS | ||||
| ISSN: | 0006-291X | ||||
| Official Date: | 30 August 1994 | ||||
| Dates: |
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| Volume: | 203 | ||||
| Number: | 1 | ||||
| Number of Pages: | 8 | ||||
| Page Range: | pp. 623-630 | ||||
| Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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