Haering, Christian H., Farcas, Ana-Maria, Arumugam, Prakash, Metson, Jean and Nasmyth, Kim (2008) The cohesin ring concatenates sister DNA molecules. Nature, Vol.454 . pp. 297-301. doi:10.1038/nature07098

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Abstract

Sister chromatid cohesion, which is essential for mitosis, is mediated by a multi-subunit
protein complex called cohesin whose Scc1, Smc1, and Smc3 subunits form a tripartite
ring structure. It has been proposed that cohesin holds sister DNAs together by trapping
them inside its ring. To test this, we used site-specific cross-linking to create chemical
connections at the three interfaces between the ring’s three constituent polypeptides,
thereby creating covalently closed cohesin rings. As predicted by the ring entrapment
model, this procedure produces dimeric DNA/cohesin structures that are resistant to
protein denaturation. We conclude that cohesin rings concatenate individual sister
minichromosome DNAs.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	DNA
Journal or Publication Title:	Nature
Publisher:	Nature Publishing
ISSN:	0028-0836
Official Date:	2 July 2008
Dates:	Date Event 2 July 2008 Published
Volume:	Vol.454
Page Range:	pp. 297-301
DOI:	10.1038/nature07098
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Description:	Version accepted by publisher (post-print, after peer review, before copy-editing).
Funder:	Medical Research Council (Great Britain) (MRC), Wellcome Trust (London, England), Cancer Research UK (CRUK)

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