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EFFICIENT BUT ABERRANT CLEAVAGE OF MITOCHONDRIAL PRECURSOR PROTEINS BY THE CHLOROPLAST STROMAL PROCESSING PEPTIDASE
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UNSPECIFIED (1994) EFFICIENT BUT ABERRANT CLEAVAGE OF MITOCHONDRIAL PRECURSOR PROTEINS BY THE CHLOROPLAST STROMAL PROCESSING PEPTIDASE. EUROPEAN JOURNAL OF BIOCHEMISTRY, 221 (1). pp. 523-528.
Research output not available from this repository, contact author.Abstract
Cytosol-synthesised chloroplast and mitochondrial precursor proteins are proteolytically processed after import by highly specific, metal-dependent soluble enzymes: the stromal processing peptidase (SPP) and the matrix processing peptidase (MPP), respectively. We have used in vitro processing assays to compare the reaction specificities of highly purified preparations of pea SPP and Neurospora crassa MPP, both of which are unable to cleave a variety of 'foreign' proteins. We show that SPP can cleave all five mitochondrial precursor proteins tested, namely cyclophilin, the beta subunit of the F-1-ATPase complex, the Rieske FeS protein, the alpha-MPP subunit and cytochrome b(2). In contrast, MPP is unable to cleave any chloroplast precursor proteins tested. Several of the mitochondrial precursor proteins are cleaved more efficiently by SPP than are many authentic chloroplast precursor proteins but, in each case, cleavage takes place at a site or sites which are N-terminal to the authentic MPP site; pre-cyclophilin is cleaved 5 residues upstream of the MPP site and the precursor of the beta subunit of the F-1-ATPase complex is cleaved at sites 5 and 12 residues upstream. We discuss the implications of these data for the SPP reaction mechanism.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry | ||||
| Journal or Publication Title: | EUROPEAN JOURNAL OF BIOCHEMISTRY | ||||
| Publisher: | SPRINGER VERLAG | ||||
| ISSN: | 0014-2956 | ||||
| Official Date: | 1 April 1994 | ||||
| Dates: |
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| Volume: | 221 | ||||
| Number: | 1 | ||||
| Number of Pages: | 6 | ||||
| Page Range: | pp. 523-528 | ||||
| Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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