UNSPECIFIED. (1994) EXPRESSION OF IMMUNOGLOBULIN HEAVY-CHAIN RICIN-A CHAIN FUSIONS IN MAMMALIAN-CELLS. MOLECULAR IMMUNOLOGY, 31 (2). pp. 117-125. ISSN 0161-5890

Abstract

Mammalian cell lines were transfected with antibody heavy (H) chain-ricin A chain gene fusions in attempts to assemble a recombinant immunotoxin. We found that a light chain-secreting mouse plasmacytoma cell line can be transfected stably with such a chimaeric gene, but only if the ricin A chain portion is disarmed by genetic means prior to transfection; if not, stable transfection appears to select for genetic inactivation of the transfected gene. Co-expression of an antibody heavy chain-ricin A chain fusion with light chain in non-lymphoid cells results in cell death. We conclude that the ricin A chain moiety retains biological activity precluding the expression of biologically active antibody-ricin A chain fusion proteins in mammalian cells.

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