UNSPECIFIED. (1994) EXPRESSION OF IMMUNOGLOBULIN HEAVY-CHAIN RICIN-A CHAIN FUSIONS IN MAMMALIAN-CELLS. MOLECULAR IMMUNOLOGY, 31 (2). pp. 117-125. ISSN 0161-5890

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Abstract

Mammalian cell lines were transfected with antibody heavy (H) chain-ricin A chain gene fusions in attempts to assemble a recombinant immunotoxin. We found that a light chain-secreting mouse plasmacytoma cell line can be transfected stably with such a chimaeric gene, but only if the ricin A chain portion is disarmed by genetic means prior to transfection; if not, stable transfection appears to select for genetic inactivation of the transfected gene. Co-expression of an antibody heavy chain-ricin A chain fusion with light chain in non-lymphoid cells results in cell death. We conclude that the ricin A chain moiety retains biological activity precluding the expression of biologically active antibody-ricin A chain fusion proteins in mammalian cells.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology > QR180 Immunology
Journal or Publication Title:	MOLECULAR IMMUNOLOGY
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
ISSN:	0161-5890
Date:	February 1994
Volume:	31
Number:	2
Number of Pages:	9
Page Range:	pp. 117-125
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/20833

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