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EXPRESSION OF IMMUNOGLOBULIN HEAVY-CHAIN RICIN-A CHAIN FUSIONS IN MAMMALIAN-CELLS
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UNSPECIFIED (1994) EXPRESSION OF IMMUNOGLOBULIN HEAVY-CHAIN RICIN-A CHAIN FUSIONS IN MAMMALIAN-CELLS. MOLECULAR IMMUNOLOGY, 31 (2). pp. 117-125.
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Abstract
Mammalian cell lines were transfected with antibody heavy (H) chain-ricin A chain gene fusions in attempts to assemble a recombinant immunotoxin. We found that a light chain-secreting mouse plasmacytoma cell line can be transfected stably with such a chimaeric gene, but only if the ricin A chain portion is disarmed by genetic means prior to transfection; if not, stable transfection appears to select for genetic inactivation of the transfected gene. Co-expression of an antibody heavy chain-ricin A chain fusion with light chain in non-lymphoid cells results in cell death. We conclude that the ricin A chain moiety retains biological activity precluding the expression of biologically active antibody-ricin A chain fusion proteins in mammalian cells.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology > QR180 Immunology |
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| Journal or Publication Title: | MOLECULAR IMMUNOLOGY | ||||
| Publisher: | PERGAMON-ELSEVIER SCIENCE LTD | ||||
| ISSN: | 0161-5890 | ||||
| Official Date: | February 1994 | ||||
| Dates: |
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| Volume: | 31 | ||||
| Number: | 2 | ||||
| Number of Pages: | 9 | ||||
| Page Range: | pp. 117-125 | ||||
| Publication Status: | Published |
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