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LIGHT-INDUCED CAROTENOGENESIS IN MYXOCOCCUS-XANTHUS - DNA-SEQUENCE ANALYSIS OF THE CARR REGION

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UNSPECIFIED (1993) LIGHT-INDUCED CAROTENOGENESIS IN MYXOCOCCUS-XANTHUS - DNA-SEQUENCE ANALYSIS OF THE CARR REGION. MOLECULAR MICROBIOLOGY, 10 (4). pp. 713-735. ISSN 0950-382X

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Abstract

The carR region encodes a light-inducible promoter, a negative regulator of the promoter and a trans-acting activator that controls the light-inducible Myxococcus xanthus carotenoid biosynthesis regulon. DNA sequence analysis revealed, downstream of the promoter, three translationally coupled genes, carQ, carR and carS. Sequencing of mutations demonstrated that carR encoded the negative regulator and was an integral membrane protein. Mutant construction and sequencing revealed that carS was the trans-acting activator and that carQ was a positive regulator of the promoter. Neither gene encodes proteins with known sequence-specific DNA-binding motifs. The sequence of the light-inducible promoter region, identified by primer extension analysis, showed similarity to the consensus sequence of the Escherichia coli stress response ('heat-shock') promoters.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Journal or Publication Title: MOLECULAR MICROBIOLOGY
Publisher: BLACKWELL SCIENCE LTD
ISSN: 0950-382X
Date: November 1993
Volume: 10
Number: 4
Number of Pages: 23
Page Range: pp. 713-735
Publication Status: Published
URI: http://wrap.warwick.ac.uk/id/eprint/20966

Data sourced from Thomson Reuters' Web of Knowledge

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