The Library
A NOVEL STRATEGY FOR THE ISOLATION OF LUXI HOMOLOGS - EVIDENCE FOR THE WIDESPREAD DISTRIBUTION OF A LUXR LUXI SUPERFAMILY IN ENTERIC BACTERIA
Tools
UNSPECIFIED (1993) A NOVEL STRATEGY FOR THE ISOLATION OF LUXI HOMOLOGS - EVIDENCE FOR THE WIDESPREAD DISTRIBUTION OF A LUXR LUXI SUPERFAMILY IN ENTERIC BACTERIA. MOLECULAR MICROBIOLOGY, 10 (3). pp. 511-520. ISSN 0950-382X.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Abstract
The pheromone N-(3-oxohexanoyl)-L-homoserine lactone (OHHL) regulates expression of bioluminescence in the marine bacterium Vibrio fischeri, the production of carbapenem antibiotic in Erwinia carotovora and exoenzymes in both E. carotovora and Pseudomonas aeruginosa. A characteristic feature of this regulatory mechanism in V. fischeri is that it is coll density-dependent, reflecting the need to accumulate sufficient pheromone to trigger the induction of gene expression. Using a lux plasmid-based bioluminescent sensor for OHHL, pheromone production by E. carotovora, Enterobacter agglomerans, Hafnia alvei, Rahnella aquatilis and Serratia marcescens has been demonstrated and shown also to be cell density-dependent. Production of OHHL implies the presence in these bacteria of a gene equivalent to luxI. Chromosomal banks from all five enteric bacteria have yielded clones capable of eliciting OHHL production when expressed in Escherichia coli. The luxI homologue from both E. carotovora (carl) and E. agglomerans (eagl) were characterized at the DNA sequence level and the deduced protein sequences have only 25% identity with the V. fischeri Luxl. Despite this, carl, eagl and luxl are shown to be biologically equivalent. An insertion mutant of eagl demonstrates that this gene is essential for OHHL production in E. agglomerans.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology |
||||
Journal or Publication Title: | MOLECULAR MICROBIOLOGY | ||||
Publisher: | BLACKWELL SCIENCE LTD | ||||
ISSN: | 0950-382X | ||||
Official Date: | November 1993 | ||||
Dates: |
|
||||
Volume: | 10 | ||||
Number: | 3 | ||||
Number of Pages: | 10 | ||||
Page Range: | pp. 511-520 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |