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THE F(1)F(0)-ATPASE BINDING-SITE OF DIBUTYLTIN-3-HYDROXYFLAVONE - INTERACTIONS WITH VENTURICIDIN, OLIGOMYCIN AND DCCD
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UNSPECIFIED (1993) THE F(1)F(0)-ATPASE BINDING-SITE OF DIBUTYLTIN-3-HYDROXYFLAVONE - INTERACTIONS WITH VENTURICIDIN, OLIGOMYCIN AND DCCD. APPLIED ORGANOMETALLIC CHEMISTRY, 7 (6). pp. 401-406. ISSN 0268-2605.
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Abstract
Dibutyltin-3-hydroxyflavone bromiide, Bu2Sn(of), is a fluorescent probe inhibitor of mitochondrial F1F0ATPase which reacts with and titrates a component of F0 with marked fluorescence enhancement and reacts similarly with chloroplast CF1CF0 and V-ATPases. Its use to monitor the interactions of other F0 inhibitors (venturicidin, oligomycin, DCCD) with F1F0ATPase, both membrane-bound and purified by solubilization is described. Trialkyltins (Bu3SnCl) back-titrate all Bu2Sn(of) interaction sites; whereas the macrolide inhibitor venturicidin backtitrates 60+/-5% and oligomycin only 30+/-3% of Bu2Sn(of) interaction sites. Bafilomycin, the macrolide inhibitor of V-ATPases, is inactive in this assay. DCCD acts in a different fashion from the other inhibitors. Current and potential applications of this fluorescent probe in mitochondrial bioenergetics and biogenesis are discussed.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | APPLIED ORGANOMETALLIC CHEMISTRY | ||||
Publisher: | JOHN WILEY & SONS LTD | ||||
ISSN: | 0268-2605 | ||||
Official Date: | October 1993 | ||||
Dates: |
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Volume: | 7 | ||||
Number: | 6 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 401-406 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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