UNSPECIFIED (1993) THE F(1)F(0)-ATPASE BINDING-SITE OF DIBUTYLTIN-3-HYDROXYFLAVONE - INTERACTIONS WITH VENTURICIDIN, OLIGOMYCIN AND DCCD. APPLIED ORGANOMETALLIC CHEMISTRY, 7 (6). pp. 401-406. ISSN 0268-2605

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Abstract

Dibutyltin-3-hydroxyflavone bromiide, Bu2Sn(of), is a fluorescent probe inhibitor of mitochondrial F1F0ATPase which reacts with and titrates a component of F0 with marked fluorescence enhancement and reacts similarly with chloroplast CF1CF0 and V-ATPases. Its use to monitor the interactions of other F0 inhibitors (venturicidin, oligomycin, DCCD) with F1F0ATPase, both membrane-bound and purified by solubilization is described. Trialkyltins (Bu3SnCl) back-titrate all Bu2Sn(of) interaction sites; whereas the macrolide inhibitor venturicidin backtitrates 60+/-5% and oligomycin only 30+/-3% of Bu2Sn(of) interaction sites. Bafilomycin, the macrolide inhibitor of V-ATPases, is inactive in this assay. DCCD acts in a different fashion from the other inhibitors. Current and potential applications of this fluorescent probe in mitochondrial bioenergetics and biogenesis are discussed.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry
Journal or Publication Title:	APPLIED ORGANOMETALLIC CHEMISTRY
Publisher:	JOHN WILEY & SONS LTD
ISSN:	0268-2605
Date:	October 1993
Volume:	7
Number:	6
Number of Pages:	6
Page Range:	pp. 401-406
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/21018

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