UNSPECIFIED. (1993) IMMUNOGENICITY AND PATHOGENICITY OF A TRIPLE TEMPERATURE-SENSITIVE MODIFIED RESPIRATORY SYNCYTIAL VIRUS IN ADULT VOLUNTEERS. VACCINE, 11 (4). pp. 473-478. ISSN 0264-410X

Abstract

The immunogenicity and pathogenicity of a strain of respiratory syncytial (RS) virus modified by sequential induction of three temperature-sensitive (ts) mutations have been evaluated by intranasal administration to 22 adult volunteers. This modified virus, a triple ts mutant designated ts1C, was derived from a double mutant ts1B evaluated in a previous trial. The original isolate (strain RSS-2) and all its derivatives were propagated throughout in human diploid cells in a specially assigned laboratory. The triple mutant ts1C is unable to multiply in MRC-5 cells at 37-degrees-C and above. Following nasal administration of ts1C, immune responses were observed in volunteers with low pre-existing neutralizing antibody titres. The ability of mutant ts1C to induce upper respiratory tract disease in adults was greatly diminished in comparison with the non-ts wild-type virus, but not markedly more so than a previously, tested double ts mutant (ts1B) which replicates at 37-degrees-C. Mutant ts1C, however, may have greater potential as a live vaccine in view of its inherently greater genetic stability.

Data sourced from Thomson Reuters' Web of Knowledge

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