UNSPECIFIED (1993) COLLISION-INDUCED DISSOCIATION OF ALKALI-METAL CATIONIZED AND PERMETHYLATED OLIGOSACCHARIDES - INFLUENCE OF THE COLLISION ENERGY AND OF THE COLLISION GAS FOR THE ASSIGNMENT OF LINKAGE POSITION. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 4 (3). pp. 197-203. ISSN 1044-0305

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Abstract

Tandem mass spectrometry has been used to study the collision-induced decomposition of [M + Na]+ ions of permethylated oligosaccharides. It is shown that many linkage positions in one compound may be determined by the presence or absence, in a single spectrum, of specific fragment ions that arise from the cleavage of two ring bonds and that the yield of such ions depends strongly on the collision energy and nature of the collision gas. In contrast to the behavior of monolithiated native oligosaccharides, the collision-induced decomposition of the sodiated and permethylated oligosaccharide samples at low energy leads to preferential cleavage of glycosidic linkages. At high collision energies, the fragment ions formed by cleavage of more than one bond are greatly enhanced, especially when helium is replaced by argon as the collision gas. Furthermore, argon is the more efficient collision gas in inducing fragmentation of the precursor ions. As an example of the application of this method, the discrimination between the 1 --> 3 and 1 --> 6-linked mannose residues in the common core of N-glycans is described.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QC Physics
Journal or Publication Title:	JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Publisher:	ELSEVIER SCIENCE INC
ISSN:	1044-0305
Date:	March 1993
Volume:	4
Number:	3
Number of Pages:	7
Page Range:	pp. 197-203
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/21503

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