Noble, Ralph, Fermor, T. R., Lincoln, Suzanne, Dobrovin-Pennington, Andreja, Evered, Carol, Mead, A. (Andrew) and Li, R.. (2003) Primordia initiation of mushroom (Agaricus bisporus) strains on axenic casing materials. Mycologia, Vol.95 (No.4). pp. 620-629. ISSN 0027-5514

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Abstract

The mushroom (Agaricus bisporus) has a requirement for a ‘‘casing layer’’ that has specific physical, chemical and microbiological properties which stimulate and promote the initiation of primordia. Some of these primordia then may develop further into sporophores, involving differentiation of tissue. Wild and commercial strains of A. bisporus were cultured in axenic and nonaxenic microcosms, using a rye grain substrate covered by a range of organic and inorganic casing materials. In axenic culture, A. bisporus (commercial strain A15) was capable of producing primordia and mature sporophores on charcoal (wood and activated), anthracite coal, lignite and zeolite, but not on bark, coir, peat, rockwool, silica or vermiculite. Of six strains tested, only the developmental variant mutant, B430, produced rudimentary primordia on axenic peat-based casing material. However, none of these rudimentary primordia developed differentiated tissues or beyond 4 mm diameter, either on axenic casing material in the microcosms or in larger-scale culture. In larger-scale, nonaxenic culture, strain B430 produced severely malformed but mature sporophores in similar numbers to those of other strains. Typically, 3–6% of primordia developed into mature sporophores, but significant differences in this proportion, as well as in the numbers of primordia produced, were recorded between 12 A. bisporus strains.

Item Type:	Journal Article
Subjects:	S Agriculture > SB Plant culture
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Cultivated mushroom -- Research, Plant cells and tissues -- Growth, Fungi -- Spores, Fungi -- Hyphae, Mycelium, Mushroom culture -- Great Britain
Journal or Publication Title:	Mycologia
Publisher:	Mycological Society of America
ISSN:	0027-5514
Date:	July 2003
Volume:	Vol.95
Number:	No.4
Page Range:	pp. 620-629
Identification Number:	10.2307/3761938
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA)
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URI:	http://wrap.warwick.ac.uk/id/eprint/2156

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