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Primordia initiation of mushroom (Agaricus bisporus) strains on axenic casing materials

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Noble, Ralph, Fermor, T. R., Lincoln, Suzanne, Dobrovin-Pennington, Andreja, Evered, Carol, Mead, A. (Andrew) and Li, R.. (2003) Primordia initiation of mushroom (Agaricus bisporus) strains on axenic casing materials. Mycologia, Vol.95 (No.4). pp. 620-629. ISSN 0027-5514

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Official URL: http://dx.doi.org/10.2307/3761938

Abstract

The mushroom (Agaricus bisporus) has a
requirement for a ‘‘casing layer’’ that has specific
physical, chemical and microbiological properties
which stimulate and promote the initiation of primordia.
Some of these primordia then may develop
further into sporophores, involving differentiation of
tissue. Wild and commercial strains of A. bisporus
were cultured in axenic and nonaxenic microcosms,
using a rye grain substrate covered by a range of organic
and inorganic casing materials. In axenic culture,
A. bisporus (commercial strain A15) was capable
of producing primordia and mature sporophores on
charcoal (wood and activated), anthracite coal, lignite
and zeolite, but not on bark, coir, peat, rockwool,
silica or vermiculite. Of six strains tested, only
the developmental variant mutant, B430, produced
rudimentary primordia on axenic peat-based casing
material. However, none of these rudimentary primordia
developed differentiated tissues or beyond 4
mm diameter, either on axenic casing material in the
microcosms or in larger-scale culture. In larger-scale,
nonaxenic culture, strain B430 produced severely
malformed but mature sporophores in similar numbers
to those of other strains. Typically, 3–6% of primordia
developed into mature sporophores, but significant
differences in this proportion, as well as in
the numbers of primordia produced, were recorded
between 12 A. bisporus strains.

Item Type:

Journal Article

Subjects:

S Agriculture > SB Plant culture

Divisions:

Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)

Library of Congress Subject Headings (LCSH):

Cultivated mushroom -- Research, Plant cells and tissues -- Growth, Fungi -- Spores, Fungi -- Hyphae, Mycelium, Mushroom culture -- Great Britain

Journal or Publication Title:

Mycologia

Publisher:

Mycological Society of America

ISSN:

0027-5514

Official Date:

July 2003

Dates:

Date	Event
July 2003	Published

Volume:

Vol.95

Number:

No.4

Page Range:

pp. 620-629

Identification Number:

10.2307/3761938

Status:

Peer Reviewed

Access rights to Published version:

Open Access

Funder:

Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA)

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