A RAPID LUMINESCENT-PHAGE BASED MPN METHOD FOR THE ENUMERATION OF SALMONELLA-TYPHIMURIUM IN ENVIRONMENTAL-SAMPLES
UNSPECIFIED. (1993) A RAPID LUMINESCENT-PHAGE BASED MPN METHOD FOR THE ENUMERATION OF SALMONELLA-TYPHIMURIUM IN ENVIRONMENTAL-SAMPLES. LETTERS IN APPLIED MICROBIOLOGY, 16 (1). pp. 24-27. ISSN 0266-8254Full text not available from this repository.
Materials such as soils, waters, sewage sludges and foods can contain low numbers of salmonellas. A most-probable-number (MPN) method that utilized a bioluminescent-bacteriophage is described that allowed the specific determination of as few as one Salmonella typhimurium cell/100 ml of material within 24 h. The method was developed with soil, lake water and sewage sludge inoculated with Salm. typhimurium and had an efficiency of 100% when tested against a traditional MPN method. The protocol is rapid, sensitive, inexpensive, has a low operator time compared to the traditional MPN method, allows for the repair of injured cells and is amenable to automation.
|Item Type:||Journal Article|
|Subjects:||T Technology > TP Chemical technology
Q Science > QR Microbiology
|Journal or Publication Title:||LETTERS IN APPLIED MICROBIOLOGY|
|Publisher:||BLACKWELL SCIENCE LTD|
|Number of Pages:||4|
|Page Range:||pp. 24-27|
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