UNSPECIFIED (1993) A RAPID LUMINESCENT-PHAGE BASED MPN METHOD FOR THE ENUMERATION OF SALMONELLA-TYPHIMURIUM IN ENVIRONMENTAL-SAMPLES. LETTERS IN APPLIED MICROBIOLOGY, 16 (1). pp. 24-27. ISSN 0266-8254

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Abstract

Materials such as soils, waters, sewage sludges and foods can contain low numbers of salmonellas. A most-probable-number (MPN) method that utilized a bioluminescent-bacteriophage is described that allowed the specific determination of as few as one Salmonella typhimurium cell/100 ml of material within 24 h. The method was developed with soil, lake water and sewage sludge inoculated with Salm. typhimurium and had an efficiency of 100% when tested against a traditional MPN method. The protocol is rapid, sensitive, inexpensive, has a low operator time compared to the traditional MPN method, allows for the repair of injured cells and is amenable to automation.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology
Journal or Publication Title:	LETTERS IN APPLIED MICROBIOLOGY
Publisher:	BLACKWELL SCIENCE LTD
ISSN:	0266-8254
Date:	January 1993
Volume:	16
Number:	1
Number of Pages:	4
Page Range:	pp. 24-27
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/21608

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