UNSPECIFIED (1992) DEVELOPMENT OF CDNA PROBES FOR TYPING GROUP-A BOVINE ROTAVIRUSES ON THE BASIS OF VP4 SPECIFICITY. [Journal Item]

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Abstract

Dot and Northern (RNA) blot hybridization assays were developed for the P typing of group A bovine rotaviruses (BRV) by using cDNA probes prepared from gene segment 4. The probes were prepared by polymerase chain reaction amplification of hyperdivergent regions (nucleotides 211 to 686) of BRV strain UK, IND, NCDV, and Cr VP4 cDNA by using specific oligonucleotide primers. The probes were P type specific (VP4) and exhibited little or no cross-reactivity with double-stranded RNA from heterologous rotavirus P types. Our studies indicate that at least three P types, as defined by polymerase chain reaction-derived VP4 gene probes from the UK, NCDV, and Cr strains, exist among the seven BRV isolates tested.

Item Type:	Journal Item
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	JOURNAL OF CLINICAL MICROBIOLOGY
Publisher:	AMER SOC MICROBIOLOGY
ISSN:	0095-1137
Date:	October 1992
Volume:	30
Number:	10
Number of Pages:	5
Page Range:	pp. 2717-2721
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/21787

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