UNSPECIFIED. (1992) 3' TERMINAL NUCLEOTIDE-SEQUENCE OF HUMAN ASTROVIRUS TYPE-1 AND ROUTINE DETECTION OF ASTROVIRUS NUCLEIC-ACID AND ANTIGENS. JOURNAL OF VIROLOGICAL METHODS, 39 (1-2). pp. 217-225. ISSN 0166-0934

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Abstract

Human astrovirus type 1 was purified by caesium chloride density-gradient centrifugation and the virus was located using an immunodot blot technique with polyclonal rabbit serum, which reacted with all five serotypes. The virus banded with a density of 1.33 g/ml. RNA was extracted from the purified virus, converted into double-stranded cDNA, using an oligo(dT) primer, and cloned into plasmid and M13 vectors. The sequence of the 3' end of astrovirus RNA adjacent to the poly(A) tract was determined. This sequence showed no significant homology with the equivalent region of other positive-sense RNA viruses. Synthetic oligonucleotide primers were designed to amplify specifically astrovirus type 1 RNA in a polymerase chain reaction.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title:	JOURNAL OF VIROLOGICAL METHODS
Publisher:	ELSEVIER SCIENCE BV
ISSN:	0166-0934
Date:	September 1992
Volume:	39
Number:	1-2
Number of Pages:	9
Page Range:	pp. 217-225
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/21788

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