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BIOTRANSFORMATIONS IN THE PEPTIDE AND CARBOHYDRATE FIELDS
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UNSPECIFIED (1992) BIOTRANSFORMATIONS IN THE PEPTIDE AND CARBOHYDRATE FIELDS. In: INTERNATIONAL SYMP ON ENZYMES IN ORGANIC SYNTHESIS, NEW DELHI, INDIA, JAN 06-09, 1992. Published in: PURE AND APPLIED CHEMISTRY, 64 (8). pp. 1079-1084. ISSN 0033-4545.
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Abstract
Proteases and glycosidases are classes of hydrolytic enzymes that are used in biotransformations to catalyse the synthesis of peptides, and of glycosides and oligosaccharaides, respectively, by a reversal of their normal mode of action. The practical application of both methods shares several common features, namely the shift of rate-determining step through the use of activated substrates (aminoacyl and glycosyl donors respectively), the need to reduce or eliminate competing hydrolysis in the aminoacyl and glycosyl transfer steps, and the need to minimise hydrolytic cleavage of the products. Control of these factors is illustrated by a method for peptide synthesis using alpha-chymotrypsin suspended in dichloromethane containing low concentrations of water and a method for disaccharide synthesis using an N-acetylgalactosaminidase from Aspergillus oryzae. Molecular modelling of the oxyanion intermediate has been found to explain a number of features of reactions catalysed by alpha-chymotrypsin and to predict others that were the subject of experimental test. The concept of "anomeric control" was used to modulate the regioselectivity of N-acetylgalactosaminyl transfer catalysed by the N-acetylgalactosaminidase.
Item Type: | Conference Item (UNSPECIFIED) | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | PURE AND APPLIED CHEMISTRY | ||||
Publisher: | BLACKWELL SCIENCE LTD | ||||
ISSN: | 0033-4545 | ||||
Official Date: | August 1992 | ||||
Dates: |
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Volume: | 64 | ||||
Number: | 8 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 1079-1084 | ||||
Publication Status: | Published | ||||
Title of Event: | INTERNATIONAL SYMP ON ENZYMES IN ORGANIC SYNTHESIS | ||||
Location of Event: | NEW DELHI, INDIA | ||||
Date(s) of Event: | JAN 06-09, 1992 |
Data sourced from Thomson Reuters' Web of Knowledge
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