UNSPECIFIED (1992) GENE-TRANSFER BETWEEN STREPTOMYCETES IN SOIL. In: 8TH INTERNATIONAL SYMP ON BIOLOGY OF ACTINOMYCETES ( ISBA-8 ), AUG 11-16, 1991, UNIV WISCONSIN, MADISON, WI.

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Abstract

The growth and activity of Streptomyces violaceolatus and Streptomyces lividans was studied in soil under controlled conditions. The life cycle was followed under differing nutrient regimes and the fate of plasmid- and phage-borne genes determined by direct and indirect techniques. Methods were developed for the direct monitoring of plasmid DNA extracted from soil which allowed differentiation of the cellular location of plasmid DNA between mycelium and spores. In a dynamic, nutrient-fed soil microcosm, inoculants survived poorly, but a specific stage was defined by direct and indirect methods when the inoculants were most active and this correlated with the detection of gene transfer events. Plasmid transfer, phage infection and lysogeny only occurred to a significant extent within this stage at days 15-17 during a 60-day incubation. Estimates based on plasmid DNA recovery indicated that viable counts underestimated spore and mycelial propagules by a factor of > 100.

Item Type:	Conference Item (UNSPECIFIED)
Subjects:	Q Science > QH Natural history > QH426 Genetics
Journal or Publication Title:	GENE
Publisher:	ELSEVIER SCIENCE BV
ISSN:	0378-1119
Date:	15 June 1992
Volume:	115
Number:	1-2
Number of Pages:	6
Page Range:	pp. 193-198
Publication Status:	Published
Title of Event:	8TH INTERNATIONAL SYMP ON BIOLOGY OF ACTINOMYCETES ( ISBA-8 )
Location of Event:	UNIV WISCONSIN, MADISON, WI
Date(s) of Event:	AUG 11-16, 1991
URI:	http://wrap.warwick.ac.uk/id/eprint/21965

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