Didelot, Xavier, Urwin, Rachel, Maiden, Martin C. J. and Falush, Daniel. (2009) Genealogical typing of Neisseria meningitidis. Microbiology, Vol.15 (No.10). pp. 3176-3186. ISSN 1350-0872

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Abstract

Despite the increasing popularity of multilocus sequence typing (MLST), the most appropriate method for characterizing bacterial variation and facilitating epidemiological investigations remains a matter of debate. Here, we propose that different typing schemes should be compared on the basis of their power to infer clonal relationships and investigate the utility of sequence data for genealogical reconstruction by exploiting new statistical tools and data from 20 housekeeping loci for 93 isolates of the bacterial pathogen Neisseria meningitidis. Our analysis demonstrated that all but one of the hyperinvasive isolates established by multilocus enzyme electrophoresis and MLST were grouped into one of six genealogical lineages, each of which contained substantial variation. Due to the confounding effect of recombination, evolutionary relationships among these lineages remained unclear, even using 20 loci. Analyses of the seven loci in the standard MLST scheme using the same methods reproduced this classification, but were unable to support finer inferences concerning the relationships between the members within each complex.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science > Statistics
Library of Congress Subject Headings (LCSH):	Neisseria meningitidis -- Research, Bacteria -- Molecular genetics, Gene mapping -- Data processing, Bioinformatics -- Research
Journal or Publication Title:	Microbiology
Publisher:	Society for General Microbiology
ISSN:	1350-0872
Date:	October 2009
Volume:	Vol.15
Number:	No.10
Page Range:	pp. 3176-3186
Identification Number:	10.1099/mic.0.031534-0
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Wellcome Trust (London, England)
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URI:	http://wrap.warwick.ac.uk/id/eprint/2198

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