UNSPECIFIED (1992) THE FATE OF INTRODUCED STREPTOMYCETES, PLASMID AND PHAGE POPULATIONS IN A DYNAMIC SOIL SYSTEM. JOURNAL OF GENERAL MICROBIOLOGY, 138 (Part 4). pp. 659-666. ISSN 0022-1287

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Abstract

Populations of Streptomyces lividans and S. violaceolatus were monitored in natural soil amended with nutrients. The fates of a multicopy plasmid pIJ673, and an actinophage, KC301, were determined and the extent of gene transfer was estimated. The soil was incubated for 60 d during which time 'spent' soil was periodically removed followed by addition of fresh, uninoculated soil. Maximum numbers of bacteria and phage inoculants occurred at 15 d; this correlated with a peak in the amount of plasmid DNA detected and total numbers of transconjugants recovered. A KC301 lysogen of S. lividans was also recovered at this time. Plasmid DNA was monitored by two methods, bead-beating and SDS/heat lysis; the latter was specific for mycelium while the former released DNA from spores and mycelium. Southern blots of soil DNA only showed the presence of plasmid DNA in SDS/heat lysis extracts from 15 d and 17 d samples, whereas positive signals were obtained throughout the experiment from bead-beaten extracts. The results confirmed that a well-developed mycelium was necessary for conjugation, phage infection, multiplication and lysogeny in soil.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	JOURNAL OF GENERAL MICROBIOLOGY
Publisher:	SOC GENERAL MICROBIOLOGY
ISSN:	0022-1287
Date:	April 1992
Volume:	138
Number:	Part 4
Number of Pages:	8
Page Range:	pp. 659-666
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/22098

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