UNSPECIFIED. (1992) ASSIGNMENT OF MUTANT TSN19 (COMPLEMENTATION GROUP-E) OF RESPIRATORY SYNCYTIAL VIRUS TO THE P-PROTEIN GENE. JOURNAL OF GENERAL VIROLOGY, 73 (Part 4). pp. 865-873. ISSN 0022-1317

Abstract

The mutation responsible for the temperature-sensitive (ts) phenotype of mutant tsN19 (complementation group E) of respiratory syncytial virus has been located to the P protein gene. Viral protein synthesis was completely restricted at 39-degrees-C, and the tsN19 P protein did not react with an anti-P monoclonal antibody (MAb) (3-5) at 33-degrees-C. Reversion of temperature sensitivity restored reactivity with MAb 3-5. Nucleotide sequence determination and in vitro expression of cDNA clones of P mRNA derived from wild-type, tsN19 and non-ts revertant-infected cells, revealed that temperature sensitivity and loss of reactivity with MAb 3-5 were consequences of a Gly --> Ser amino acid change at position 172. A low M(r) polypeptide, which represented the C-terminal 93 amino acids of the P protein, was produced by internal initiation in the P open reading frame during in vitro translation, and a similar product was detected transiently in vivo.

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