UNSPECIFIED (1992) INDUCIBLE CELL ABLATION IN DROSOPHILA BY COLD-SENSITIVE RICIN-A CHAIN. DEVELOPMENT, 114 (3). 681-&. ISSN 0950-1991

Full text not available from this repository.

Abstract

We have developed a system for temperature-inducible killing of specific cells in the fruitfly Drosophila melanogaster. The system overcomes many of the limitations of existing cell ablation methods and is in principle applicable to any non-homeothermic eukaryote. Temperature-sensitive and cold-sensitive mutations in the ricin toxin A chain (RTA) of castor bean were generated in yeast. One cold-sensitive mutation, RAcs2, produced temperature-dependent ablation of eye cells in Drosophila when expressed under control of the eye-specific sev enhancer. At 29-degrees-C, cell death was observed within 7 hours in the developing eye and no obvious toxic effects were observed elsewhere; at 18-degrees-C, extremely low toxicity was observed. DNA sequencing of RAcs2 revealed a single amino acid substitution in the RTA active site cleft.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology
Journal or Publication Title:	DEVELOPMENT
Publisher:	COMPANY OF BIOLOGISTS LTD
ISSN:	0950-1991
Date:	March 1992
Volume:	114
Number:	3
Number of Pages:	0
Page Range:	681-&
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/22141

Data sourced from Thomson Reuters' Web of Knowledge

Request changes to a record

Actions (login required)

View Item