BIOLOGICAL-ACTIVITY OF RECOMBINANT RICINUS-COMMUNIS AGGLUTININ A-CHAIN PRODUCED IN ESCHERICHIA-COLI
UNSPECIFIED. (1992) BIOLOGICAL-ACTIVITY OF RECOMBINANT RICINUS-COMMUNIS AGGLUTININ A-CHAIN PRODUCED IN ESCHERICHIA-COLI. FEBS LETTERS, 299 (3). pp. 209-212. ISSN 0014-5793Full text not available from this repository.
DNA encoding Ricinus communis agglutinin A chain was ligated into the E coli expression vector pDS 5/3. Induced E. coli 71.18 cells which had been transformed with this plasmid express Ricinus communis agglutinin A chain in a soluble and biologically active form. Recombinant Ricinus communis agglutinin A chain had ribosomal RNA N-glycosidase activity and was approximately 10-fold less active than ricin A chain in a cell-free protein synthesis inhibition assay.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Journal or Publication Title:||FEBS LETTERS|
|Publisher:||ELSEVIER SCIENCE BV|
|Official Date:||16 March 1992|
|Number of Pages:||4|
|Page Range:||pp. 209-212|
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