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BIOLOGICAL-ACTIVITY OF RECOMBINANT RICINUS-COMMUNIS AGGLUTININ A-CHAIN PRODUCED IN ESCHERICHIA-COLI
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UNSPECIFIED (1992) BIOLOGICAL-ACTIVITY OF RECOMBINANT RICINUS-COMMUNIS AGGLUTININ A-CHAIN PRODUCED IN ESCHERICHIA-COLI. FEBS LETTERS, 299 (3). pp. 209-212. ISSN 0014-5793.
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Abstract
DNA encoding Ricinus communis agglutinin A chain was ligated into the E coli expression vector pDS 5/3. Induced E. coli 71.18 cells which had been transformed with this plasmid express Ricinus communis agglutinin A chain in a soluble and biologically active form. Recombinant Ricinus communis agglutinin A chain had ribosomal RNA N-glycosidase activity and was approximately 10-fold less active than ricin A chain in a cell-free protein synthesis inhibition assay.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
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Journal or Publication Title: | FEBS LETTERS | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0014-5793 | ||||
Official Date: | 16 March 1992 | ||||
Dates: |
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Volume: | 299 | ||||
Number: | 3 | ||||
Number of Pages: | 4 | ||||
Page Range: | pp. 209-212 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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