UNSPECIFIED (1992) ADVANTAGES OF HIGH-RESOLUTION AND HIGH-MASS RANGE MAGNETIC-SECTOR MASS-SPECTROMETRY FOR ELECTROSPRAY IONIZATION. ORGANIC MASS SPECTROMETRY, 27 (3). pp. 195-203. ISSN 0030-493X

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Abstract

Electrospray ionization (ESI) mass spectra have been measured on a magnetic-sector double-focusing mass spectrometer for a number of proteins and peptides. It is pointed out how in theory raising the mass resolution of a mass spectrometer from 800-1000 to 2400-3000 significantly increases the precision with which the envelope of isotopic peaks of a protein ion (or other organic ion) can be defined, particularly at higher masses. Better definition of the isotopic envelope ought to lead to higher precision in the experimental determination of molecular mass, which has been demonstrated. It is shown how ESI mass spectra of high-mass molecules are significantly less congested at higher m/z values, so that for these molecules (RMM > 40 000) there is an advantage in being able to record peaks at higher m/z values (m/z > 2000) representing ions with fewer charges. Fragmentation of a small peptide in the ESI source has been found to provide sequence information.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QC Physics
Journal or Publication Title:	ORGANIC MASS SPECTROMETRY
Publisher:	JOHN WILEY & SONS LTD
ISSN:	0030-493X
Date:	March 1992
Volume:	27
Number:	3
Number of Pages:	9
Page Range:	pp. 195-203
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/22163

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