THE LECTIN GENE FAMILY OF RICINUS-COMMUNIS - CLONING OF A FUNCTIONAL RICIN GENE AND 3 LECTIN PSEUDOGENES
UNSPECIFIED. (1992) THE LECTIN GENE FAMILY OF RICINUS-COMMUNIS - CLONING OF A FUNCTIONAL RICIN GENE AND 3 LECTIN PSEUDOGENES. PLANT MOLECULAR BIOLOGY, 18 (3). pp. 515-525. ISSN 0167-4412Full text not available from this repository.
Molecular hybridisation using a ricin cDNA probe has revealed that the ricin/Ricinus communis agglutinin (RCA) multigene family is composed of approximately eight members. Several genomic clones containing preproricin and preproricin-like sequences have been isolated. Partial analysis of three different genomic clones by DNA sequencing and ribonuclease protection has indicated that at least three members of the lectin gene family are non-functional. None of the original seventeen positive clones isolated appears to contain a Ricinus communis agglutinin (RCA) gene. One gene member analysed (pCBG3H1) represents a functional ricin gene similar in coding sequence to the published cDNA sequence and possesses typical eukaryotic consensus sequences and seed-specific elements within the flanking sequences. Investigation at the transcriptional level of the expression pattern of this gene revealed that mRNA accumulates during the post-testa stages of seed development. The pattern of accumulation of steady-state transcripts correlates closely with that previously observed at the protein and translatable RNA levels.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry
S Agriculture > SB Plant culture
|Journal or Publication Title:||PLANT MOLECULAR BIOLOGY|
|Publisher:||KLUWER ACADEMIC PUBL|
|Number of Pages:||11|
|Page Range:||pp. 515-525|
Actions (login required)