EFFECT OF CHANGES IN THE NUCLEOTIDE-SEQUENCE OF THE P-GENE OF RESPIRATORY SYNCYTIAL VIRUS ON THE ELECTROPHORETIC MOBILITY OF THE P-PROTEIN
UNSPECIFIED (1992) EFFECT OF CHANGES IN THE NUCLEOTIDE-SEQUENCE OF THE P-GENE OF RESPIRATORY SYNCYTIAL VIRUS ON THE ELECTROPHORETIC MOBILITY OF THE P-PROTEIN. VIRUS GENES, 6 (1). pp. 53-62. ISSN 0920-8569Full text not available from this repository.
A consensus sequence for the P protein gene of the RSN-2 strain of respiratory syncytial (RS) virus was obtained by PCR amplification of cDNA obtained by reverse transcription. This established that the extent of sequence variation between two P genes of strains of antigenic subtype B is similar to that among A strains, confirming the conservation of P genes within subtypes and the divergence of the two antigenic subtypes of RS virus. The P protein of RS virus exhibits anomalous electrophoretic mobility with respect to its molecular weight. In vitro transcription and translation of RSN-2 strain cDNA possessing single point mutations revealed that substitutions involving charged amino acids in the carboxy-terminal region had a marked effect on the electrophoretic mobility of the P protein.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology > QR355 Virology
|Journal or Publication Title:||VIRUS GENES|
|Publisher:||KLUWER ACADEMIC PUBL|
|Number of Pages:||10|
|Page Range:||pp. 53-62|
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