TRANSPORT OF PROTEINS INTO CHLOROPLASTS - DELINEATION OF ENVELOPE TRANSIT AND THYLAKOID TRANSFER SIGNALS WITHIN THE PRE-SEQUENCES OF 3 IMPORTED THYLAKOID LUMEN PROTEINS
UNSPECIFIED. (1991) TRANSPORT OF PROTEINS INTO CHLOROPLASTS - DELINEATION OF ENVELOPE TRANSIT AND THYLAKOID TRANSFER SIGNALS WITHIN THE PRE-SEQUENCES OF 3 IMPORTED THYLAKOID LUMEN PROTEINS. JOURNAL OF BIOLOGICAL CHEMISTRY, 266 (35). pp. 23606-23610. ISSN 0021-9258Full text not available from this repository.
The targeting of cytosolically synthesized proteins into the thylakoid lumen is mediated by an aminoterminal pre-sequence consisting of an "envelope transit" and a "thylakoid transfer" signal in tandem. We have investigated the structural characteristics of several thylakoid transfer signals by determining the intermediate sites at which the stromal processing peptidase cleaves to remove the transit sequences. Using this approach we have found that the thylakoid transfer signals of Silene pratensis plastocyanin, 23-kDa oxygen-evolving complex protein from wheat, and 33-kDa oxygen-evolving complex protein from wheat, are 25, 39, and 48 residues in length, respectively. All of the transfer signals contain hydrophobic core sequences and a "-3,-1" motif reminiscent of those found in signal sequences, but the amino-terminal regions of the transfer signals of the 23- and 33-kDa proteins are both longer and more highly charged. The net charge of each amino-terminal region of the transfer sequences is +1, including the amino-terminal amino group. In each case, the stromal processing peptidase cleaves immediately after a positively charged residue, but otherwise the cleavage sites exhibit no common elements of either primary or secondary structure.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry|
|Journal or Publication Title:||JOURNAL OF BIOLOGICAL CHEMISTRY|
|Publisher:||AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC|
|Official Date:||15 December 1991|
|Number of Pages:||5|
|Page Range:||pp. 23606-23610|
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