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TARGETING OF RICIN-A CHAIN INTO PEA-CHLOROPLASTS

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UNSPECIFIED (1991) TARGETING OF RICIN-A CHAIN INTO PEA-CHLOROPLASTS. FEBS LETTERS, 293 (1-2). pp. 134-136. ISSN 0014-5793

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Abstract

A chimaeric gene was constructed encoding the pre-sequence of the 33 kDa oxygen-evolving complex protein from wheat (a thylakoid lumen protein) linked to ricin A chain. The fusion protein is efficiently imported by isolated pea chloroplasts and localised partly in the stroma, with the remainder bound to the stromal surface of the thylakoids. The imported protein is fully processed by both the stromal and thylakoidal processing peptidases, indicating that partial or complete translocation across the thylakoid membrane has taken place.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Journal or Publication Title: FEBS LETTERS
Publisher: ELSEVIER SCIENCE BV
ISSN: 0014-5793
Date: 18 November 1991
Volume: 293
Number: 1-2
Number of Pages: 3
Page Range: pp. 134-136
Publication Status: Published
URI: http://wrap.warwick.ac.uk/id/eprint/22342

Data sourced from Thomson Reuters' Web of Knowledge

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