THE METHANE MONOOXYGENASE GENE-CLUSTER OF METHYLOSINUS-TRICHOSPORIUM - CLONING AND SEQUENCING OF THE MMOC GENE
UNSPECIFIED (1991) THE METHANE MONOOXYGENASE GENE-CLUSTER OF METHYLOSINUS-TRICHOSPORIUM - CLONING AND SEQUENCING OF THE MMOC GENE. ARCHIVES OF MICROBIOLOGY, 156 (6). pp. 477-483. ISSN 0302-8933Full text not available from this repository.
Methane monooxygenase (MMO) is the enzyme responsible for the conversion of methane to methanol in methanotrophic bacteria. The soluble MMO enzyme complex from Methylosinus trichosporium also oxidizes a wide range of aliphatic and aromatic compounds in a number of potentially useful biotransformations. In this study we have used heterologous DNA probes from the type X methanotroph Methylococcus capsulatus (Bath) to isolate mmo genes from the type II methanotroph M. trichosporium. We report here that the gene encoding the reductase component, Protein C of MMO, lies adjacent to the genes encoding the other components of soluble MMO in M. trichosporium but is separated by an open reading frame of unknown function, orfY. The complete nucleotide sequence of these genes is presented. Sequence analysis of mmoC indicates that the N-terminus of Protein C has significant homology with 2Fe2S ferredoxins from a wide range of organisms.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology|
|Journal or Publication Title:||ARCHIVES OF MICROBIOLOGY|
|Number of Pages:||7|
|Page Range:||pp. 477-483|
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