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THE FULL PRECURSOR OF THE 33 KDA OXYGEN-EVOLVING COMPLEX PROTEIN OF WHEAT IS EXPORTED BY ESCHERICHIA-COLI AND PROCESSED TO THE MATURE SIZE
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UNSPECIFIED (1991) THE FULL PRECURSOR OF THE 33 KDA OXYGEN-EVOLVING COMPLEX PROTEIN OF WHEAT IS EXPORTED BY ESCHERICHIA-COLI AND PROCESSED TO THE MATURE SIZE. PLANT MOLECULAR BIOLOGY, 17 (6). pp. 1241-1243. ISSN 0167-4412
Full text not available from this repository.Abstract
A full-length cDNA encoding the precursor of the lumenal 33 kDa oxygen-evolving complex protein from wheat was inserted into a prokaryotic expression vector. Cell-free transcription-translation of this construct generates a precursor protein of the correct size. However, when expressed in Escherichia coli, the protein is quantitatively exported into the periplasm and processed to the mature size. The results indicate that the thylakoid transfer sequence of this precursor can function as an internal E. coli export signal.
| Item Type: | Journal Item | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry S Agriculture > SB Plant culture |
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| Journal or Publication Title: | PLANT MOLECULAR BIOLOGY | ||||
| Publisher: | KLUWER ACADEMIC PUBL | ||||
| ISSN: | 0167-4412 | ||||
| Official Date: | December 1991 | ||||
| Dates: |
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| Volume: | 17 | ||||
| Number: | 6 | ||||
| Number of Pages: | 3 | ||||
| Page Range: | pp. 1241-1243 | ||||
| Publication Status: | Published | ||||
| URI: | http://wrap.warwick.ac.uk/id/eprint/22368 |
Data sourced from Thomson Reuters' Web of Knowledge
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