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STRUCTURE OF THE ECDYSONE GLUCOSIDE FORMED BY A BACULOVIRUS ECDYSTEROID UDP-GLUCOSYLTRANSFERASE

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UNSPECIFIED (1991) STRUCTURE OF THE ECDYSONE GLUCOSIDE FORMED BY A BACULOVIRUS ECDYSTEROID UDP-GLUCOSYLTRANSFERASE. INSECT BIOCHEMISTRY, 21 (7). pp. 795-801. ISSN 0020-1790.

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Abstract

The egt gene of the baculovirus Autographa californica nuclear polyhedrosis virus encodes an ecdysteroid UDP-glucosyltransferase. The glucoside formed by this enzyme using ecdysone and UDP-glucose as substrates has been purified and structurally characterized by nuclear magnetic resonance spectroscopy and by fast atom bombardment mass spectrometry. These studies have identified the conjugate as ecdysone 22-O-beta-D-glucopyranoside. Substrate specificity studies have confirmed that ecdysteroids lacking a hydroxyl moiety at C-22 are not substrates for the enzyme.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QL Zoology
Journal or Publication Title: INSECT BIOCHEMISTRY
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
ISSN: 0020-1790
Official Date: 1991
Dates:
DateEvent
1991UNSPECIFIED
Volume: 21
Number: 7
Number of Pages: 7
Page Range: pp. 795-801
Publication Status: Published

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