UNSPECIFIED. (1991) STRUCTURE OF THE ECDYSONE GLUCOSIDE FORMED BY A BACULOVIRUS ECDYSTEROID UDP-GLUCOSYLTRANSFERASE. INSECT BIOCHEMISTRY, 21 (7). pp. 795-801. ISSN 0020-1790

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Abstract

The egt gene of the baculovirus Autographa californica nuclear polyhedrosis virus encodes an ecdysteroid UDP-glucosyltransferase. The glucoside formed by this enzyme using ecdysone and UDP-glucose as substrates has been purified and structurally characterized by nuclear magnetic resonance spectroscopy and by fast atom bombardment mass spectrometry. These studies have identified the conjugate as ecdysone 22-O-beta-D-glucopyranoside. Substrate specificity studies have confirmed that ecdysteroids lacking a hydroxyl moiety at C-22 are not substrates for the enzyme.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QL Zoology
Journal or Publication Title:	INSECT BIOCHEMISTRY
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
ISSN:	0020-1790
Date:	1991
Volume:	21
Number:	7
Number of Pages:	7
Page Range:	pp. 795-801
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/22371

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