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STREPTOMYCES MARKER PLASMIDS FOR MONITORING SURVIVAL AND SPREAD OF STREPTOMYCETES IN SOIL
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UNSPECIFIED (1991) STREPTOMYCES MARKER PLASMIDS FOR MONITORING SURVIVAL AND SPREAD OF STREPTOMYCETES IN SOIL. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 57 (11). pp. 3322-3330.
Research output not available from this repository, contact author.Abstract
Plamid constructs pNW1 through pNW6 containing a controllable xylE gene (for catechol 2,3-dioxygenase) were introduced into Streptomyces lividans strains to provide a selectable marker system. xylE functions in S. lividans under the control of bacteriophage lambda promoters lambda-p(L) and lambda-p(R). Thermoregulated expression of xylE is provided through the lambda repressor cI857. Catechol 2,3-dioxygenase activity was increased 2.8-fold from plasmid construct pNW2 (lambda-p(L), xylE, cI857) and 9.5- and 7.4-fold from constructs pNW3 (lambda-p(R), xylE, cI857) and pNW5 (lambda-p(R), xylE, cI857), respectively, when the temperature was shifted from 28-degrees-C to 37-degrees-C. The stability of the constructs varied from 4.7% for pNW2 to 99.4% for pNW4 (lambda-p(L), xylE) over two rounds of sporulation. Marked S. lividans strains released into soil systems retained the XylE phenotype for more than 80 days, depending on the marker plasmid, when examined by a selective plating method. Furthermore, S. lividans harboring plasmid pNW5 was detectable by nucleic acid hybridization at less than 10 CFU g-1 (dry weight) of soil as mycelium and 10(3) CFU g-1 (dry weight) of soil as spores with the xylE marker DNA extracted from soil and amplified by using the polymerase chain reaction.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology |
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| Journal or Publication Title: | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | ||||
| Publisher: | AMER SOC MICROBIOLOGY | ||||
| ISSN: | 0099-2240 | ||||
| Official Date: | November 1991 | ||||
| Dates: |
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| Volume: | 57 | ||||
| Number: | 11 | ||||
| Number of Pages: | 9 | ||||
| Page Range: | pp. 3322-3330 | ||||
| Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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