STREPTOMYCES MARKER PLASMIDS FOR MONITORING SURVIVAL AND SPREAD OF STREPTOMYCETES IN SOIL
UNSPECIFIED. (1991) STREPTOMYCES MARKER PLASMIDS FOR MONITORING SURVIVAL AND SPREAD OF STREPTOMYCETES IN SOIL. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 57 (11). pp. 3322-3330. ISSN 0099-2240Full text not available from this repository.
Plamid constructs pNW1 through pNW6 containing a controllable xylE gene (for catechol 2,3-dioxygenase) were introduced into Streptomyces lividans strains to provide a selectable marker system. xylE functions in S. lividans under the control of bacteriophage lambda promoters lambda-p(L) and lambda-p(R). Thermoregulated expression of xylE is provided through the lambda repressor cI857. Catechol 2,3-dioxygenase activity was increased 2.8-fold from plasmid construct pNW2 (lambda-p(L), xylE, cI857) and 9.5- and 7.4-fold from constructs pNW3 (lambda-p(R), xylE, cI857) and pNW5 (lambda-p(R), xylE, cI857), respectively, when the temperature was shifted from 28-degrees-C to 37-degrees-C. The stability of the constructs varied from 4.7% for pNW2 to 99.4% for pNW4 (lambda-p(L), xylE) over two rounds of sporulation. Marked S. lividans strains released into soil systems retained the XylE phenotype for more than 80 days, depending on the marker plasmid, when examined by a selective plating method. Furthermore, S. lividans harboring plasmid pNW5 was detectable by nucleic acid hybridization at less than 10 CFU g-1 (dry weight) of soil as mycelium and 10(3) CFU g-1 (dry weight) of soil as spores with the xylE marker DNA extracted from soil and amplified by using the polymerase chain reaction.
|Item Type:||Journal Article|
|Subjects:||T Technology > TP Chemical technology
Q Science > QR Microbiology
|Journal or Publication Title:||APPLIED AND ENVIRONMENTAL MICROBIOLOGY|
|Publisher:||AMER SOC MICROBIOLOGY|
|Official Date:||November 1991|
|Number of Pages:||9|
|Page Range:||pp. 3322-3330|
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