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DEFECTIVE SYNTHESIS OF ENVELOPE PROTEINS BY TEMPERATURE-SENSITIVE MUTANTS REPRESENTING COMPLEMENTATION GROUP-B AND GROUP-D OF RESPIRATORY SYNCYTIAL VIRUS

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UNSPECIFIED (1991) DEFECTIVE SYNTHESIS OF ENVELOPE PROTEINS BY TEMPERATURE-SENSITIVE MUTANTS REPRESENTING COMPLEMENTATION GROUP-B AND GROUP-D OF RESPIRATORY SYNCYTIAL VIRUS. JOURNAL OF GENERAL VIROLOGY, 72 (Part 10). pp. 2501-2508. ISSN 0022-1317

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Abstract

The phenotypes of two complementing temperature-sensitive (ts) mutants of respiratory syncytial (RS) virus indicate that the mutational lesions involve the attachment (G) and matrix (M) proteins of the viral envelope. Synthesis of the G protein was affected in cells infected with mutant tsA2 (complementation group B); the p50 precursor of the G protein was synthesized normally, but further maturation to the fully glycosylated form was defective at 39-degrees-C. A non-ts alteration in the efficiency of cleavage of the F0 precursor to the F1 and F2 subunits of the fusion protein was also observed in tsA2-infected cells, which is consistent with the aberrant non-syncytial plaque morphology induced by tsA2 in certain cells. In cells infected with mutant tsN1 (complementation group D) the M protein disappeared from the soluble cytoplasmic fraction soon after synthesis at 39-degrees-C and had a slightly decreased electrophoretic mobility. The M protein of non-ts revertants was stable at 39-degrees-C, which links the defect in M protein stability with the tsN1 phenotype. However, the aberrant mobility phenotype remained, suggesting pseudoreversion. These results assign two of the eight complementation groups of ts mutants of RS virus.

Item Type: Journal Article
Subjects: T Technology > TP Chemical technology
Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title: JOURNAL OF GENERAL VIROLOGY
Publisher: SOC GENERAL MICROBIOLOGY
ISSN: 0022-1317
Date: October 1991
Volume: 72
Number: Part 10
Number of Pages: 8
Page Range: pp. 2501-2508
Publication Status: Published
URI: http://wrap.warwick.ac.uk/id/eprint/22425

Data sourced from Thomson Reuters' Web of Knowledge

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