DEFECTIVE SYNTHESIS OF ENVELOPE PROTEINS BY TEMPERATURE-SENSITIVE MUTANTS REPRESENTING COMPLEMENTATION GROUP-B AND GROUP-D OF RESPIRATORY SYNCYTIAL VIRUS
UNSPECIFIED. (1991) DEFECTIVE SYNTHESIS OF ENVELOPE PROTEINS BY TEMPERATURE-SENSITIVE MUTANTS REPRESENTING COMPLEMENTATION GROUP-B AND GROUP-D OF RESPIRATORY SYNCYTIAL VIRUS. JOURNAL OF GENERAL VIROLOGY, 72 (Part 10). pp. 2501-2508. ISSN 0022-1317Full text not available from this repository.
The phenotypes of two complementing temperature-sensitive (ts) mutants of respiratory syncytial (RS) virus indicate that the mutational lesions involve the attachment (G) and matrix (M) proteins of the viral envelope. Synthesis of the G protein was affected in cells infected with mutant tsA2 (complementation group B); the p50 precursor of the G protein was synthesized normally, but further maturation to the fully glycosylated form was defective at 39-degrees-C. A non-ts alteration in the efficiency of cleavage of the F0 precursor to the F1 and F2 subunits of the fusion protein was also observed in tsA2-infected cells, which is consistent with the aberrant non-syncytial plaque morphology induced by tsA2 in certain cells. In cells infected with mutant tsN1 (complementation group D) the M protein disappeared from the soluble cytoplasmic fraction soon after synthesis at 39-degrees-C and had a slightly decreased electrophoretic mobility. The M protein of non-ts revertants was stable at 39-degrees-C, which links the defect in M protein stability with the tsN1 phenotype. However, the aberrant mobility phenotype remained, suggesting pseudoreversion. These results assign two of the eight complementation groups of ts mutants of RS virus.
|Item Type:||Journal Article|
|Subjects:||T Technology > TP Chemical technology
Q Science > QR Microbiology > QR355 Virology
|Journal or Publication Title:||JOURNAL OF GENERAL VIROLOGY|
|Publisher:||SOC GENERAL MICROBIOLOGY|
|Official Date:||October 1991|
|Number of Pages:||8|
|Page Range:||pp. 2501-2508|
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