Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

DETECTION AND QUANTIFICATION OF STREPTOMYCES-VIOLACEOLATUS PLASMID DNA IN SOIL

Tools
- Tools
+ Tools

UNSPECIFIED (1991) DETECTION AND QUANTIFICATION OF STREPTOMYCES-VIOLACEOLATUS PLASMID DNA IN SOIL. LETTERS IN APPLIED MICROBIOLOGY, 13 (4). pp. 193-197.

Research output not available from this repository, contact author.

Request Changes to record.

Abstract

Two methods for the direct extraction of DNA from soil were investigated using soil inoculated with Streptomyces violaceolatus ISP5438 harbouring the multicopy plasmid pIJ673. Detection limits for plasmid DNA were determined by Southern blot technique. An SDS/heat lysis method gave approximately two orders of magnitude less sensitivity than lysis and extraction by bead-beating soil inoculated with spores. The use of these two methods allowed differentiation between spore- and mycelial-borne DNA. This was due to the resistance of the spores to lysis when subjected to SDS/heat lysis.

Item Type: Journal Article
Subjects: T Technology > TP Chemical technology
Q Science > QR Microbiology
Journal or Publication Title: LETTERS IN APPLIED MICROBIOLOGY
Publisher: BLACKWELL SCIENCE LTD
ISSN: 0266-8254
Official Date: 1991
Dates:
DateEvent
1991UNSPECIFIED
Volume: 13
Number: 4
Number of Pages: 5
Page Range: pp. 193-197
Publication Status: Published

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us