UNSPECIFIED (1991) PREPROABRIN - GENOMIC CLONING, CHARACTERIZATION AND THE EXPRESSION OF THE A-CHAIN IN ESCHERICHIA-COLI. EUROPEAN JOURNAL OF BIOCHEMISTRY, 198 (3). pp. 723-732. ISSN 0014-2956

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Abstract

Synthetic oligonucleotides representing all possible sequences of an N-terminal and an internal region of the A-chain of abrin C were used to generate a probe specific for abrin-related sequences using the polymerase chain reaction on Abrus precatorius genomic DNA. A lambda phage library constructed from genomic DNA isolated from leaf tissue of A. precatorius was screened and positive hybridising clones were characterised by restriction enzyme analysis. The coding regions of unique clones were characterised by DNA sequencing. One clone encodes a preproprotein closely related to abrin C with 83% similarity between the A-chain sequences. Based on similarity with the ricin toxins and Ricinus communis agglutinin, the preproabrin consists of an A-chain of 251 amino acids preceded by 34 amino acids containing an N-terminal signal peptide, followed by a 14-amino-acid linker and a B-chain of 263 amino acids. The mature A-chain of the preproabrin has been expressed cytoplasmically in Escherichia coli and the soluble recombinant protein was produced at levels exceeding 6% of total cell protein. The recombinant A-chain has been purified to homogeneity and its ability to depurinate 28S rRNA in rat liver ribosomes has been demonstrated in vitro.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry
Journal or Publication Title:	EUROPEAN JOURNAL OF BIOCHEMISTRY
Publisher:	SPRINGER VERLAG
ISSN:	0014-2956
Date:	15 June 1991
Volume:	198
Number:	3
Number of Pages:	10
Page Range:	pp. 723-732
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/22635

Data sourced from Thomson Reuters' Web of Knowledge

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