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AN ACID-STABLE CYTOCHROME IN IRON-OXIDIZING LEPTOSPIRILLUM-FERROOXIDANS
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UNSPECIFIED (1991) AN ACID-STABLE CYTOCHROME IN IRON-OXIDIZING LEPTOSPIRILLUM-FERROOXIDANS. FEMS MICROBIOLOGY LETTERS, 81 (1). pp. 89-94. ISSN 0378-1097.
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Abstract
A novel, apparently acid-stable cytochrome has been purified from Leptospirillum ferrooxidans in which it probably functions in the 'downhill' transfer of electrons from ferrous iron. It appeared to comprise a single polypeptide of apparent M(r) 17.9 kDa as determined by SDS-PAGE. Non-denaturing PAGE showed this to be the approximate size of the native protein. The oxidized cytochrome showed a broad absorption maximum at 422 nm and was readily reduced with dithionite or ferrous iron to give a form with absorption maxima at 440 and 579 nm. A mid-point potential of +680 mV at pH 3.5 was determined. Iron and zinc were found at concentrations approaching one atom of each per cytochrome molecule.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology | ||||
| Journal or Publication Title: | FEMS MICROBIOLOGY LETTERS | ||||
| Publisher: | ELSEVIER SCIENCE BV | ||||
| ISSN: | 0378-1097 | ||||
| Official Date: | 1 June 1991 | ||||
| Dates: |
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| Volume: | 81 | ||||
| Number: | 1 | ||||
| Number of Pages: | 6 | ||||
| Page Range: | pp. 89-94 | ||||
| Publication Status: | Published |
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