AN INFLUENZA HEMAGGLUTININ-SPECIFIC IGG ENHANCES CLASS-I MHC-RESTRICTED CTL KILLING INVITRO
UNSPECIFIED. (1991) AN INFLUENZA HEMAGGLUTININ-SPECIFIC IGG ENHANCES CLASS-I MHC-RESTRICTED CTL KILLING INVITRO. IMMUNOLOGY, 73 (1). pp. 12-18. ISSN 0019-2805Full text not available from this repository.
Lungs of H-2k mice co-inoculated with type A/WSN influenza virus + detective interfering WSN virus contain haemagglutinin (HA)-specific IgG which have three different activities. These have been purified by adsorbtion and elution using different forms of HA. The first IgG recognizes HA in a form present on H-2k cells infected with a vaccinia virus recombinant expressing the WSN HA gene (vaccinia-HA virus), but not on virus particles, and enhances class I major histocompatibility complex (MHC)-restricted killing of WSN-infected H-2k target cells by primary cytotoxic T lymphocytes (CTL) from the lungs of WSN-infected H-2k mice; it also confers on primary CTL from the lungs of WSN-infected H-2d mice the ability to lyse WSN-infected H-2k targets. This IgG is therefore analogous to the T-cell receptor in that it is antigen specific and MHC restricted. A second IgG recognizes HA in a form present on both H-2k and H-2d cells infected with the vaccinia-HA virus but not present on virus particles and inhibits CTL lysis of WSN-infected syngeneic target cells. Only the third binds to virus particles; this inhibits agglutination of red cells, but is non-neutralizing. It also inhibits CTL lysis of WSN-infected syngeneic targets. Thus we present evidence that HA-specific IgG may have a significant role in regulating CTL responses to influenza virus in vivo and that one of these IgG is MHC-restricted in its recognition of viral antigen. Finally, in vivo significance of these antibodies is indicated by the finding that adoptively transferred CTL-enhancing IgG protects mice from lethal WSN infection.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology > QR180 Immunology|
|Journal or Publication Title:||IMMUNOLOGY|
|Publisher:||BLACKWELL SCIENCE LTD|
|Number of Pages:||7|
|Page Range:||pp. 12-18|
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