UNSPECIFIED (1991) CLONING OF NITROGENASE STRUCTURAL GENES FROM THE OBLIGATE METHANOTROPH METHYLOCOCCUS-CAPSULATUS (BATH). FEMS MICROBIOLOGY LETTERS, 78 (2-3). pp. 121-126. ISSN 0378-1097

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Abstract

Southern hybridization techniques were used to examine the DNA homologies between the three nitrogenase structural genes nif H, nif D and nif K of Klebsiella pneumoniae and DNA from the obligate methane oxidizing bacterium Methylococcus capsulatus (Bath). The high degree of homology between methanotroph DNA sequences and the Klebsiella nif H and nif D genes was used to isolate and clone the corresponding Methylococcus nif H and nif D genes. Subsequent restriction analysis revealed that all three nif structural genes were contiguous in the Methylococcus genome in the order nif H, nif D and nif K, as found for the majority of other diazotrophs.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	FEMS MICROBIOLOGY LETTERS
Publisher:	ELSEVIER SCIENCE BV
ISSN:	0378-1097
Date:	1 March 1991
Volume:	78
Number:	2-3
Number of Pages:	6
Page Range:	pp. 121-126
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/22798

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