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CLONING OF NITROGENASE STRUCTURAL GENES FROM THE OBLIGATE METHANOTROPH METHYLOCOCCUS-CAPSULATUS (BATH)
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UNSPECIFIED (1991) CLONING OF NITROGENASE STRUCTURAL GENES FROM THE OBLIGATE METHANOTROPH METHYLOCOCCUS-CAPSULATUS (BATH). FEMS MICROBIOLOGY LETTERS, 78 (2-3). pp. 121-126. ISSN 0378-1097.
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Abstract
Southern hybridization techniques were used to examine the DNA homologies between the three nitrogenase structural genes nif H, nif D and nif K of Klebsiella pneumoniae and DNA from the obligate methane oxidizing bacterium Methylococcus capsulatus (Bath). The high degree of homology between methanotroph DNA sequences and the Klebsiella nif H and nif D genes was used to isolate and clone the corresponding Methylococcus nif H and nif D genes. Subsequent restriction analysis revealed that all three nif structural genes were contiguous in the Methylococcus genome in the order nif H, nif D and nif K, as found for the majority of other diazotrophs.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Journal or Publication Title: | FEMS MICROBIOLOGY LETTERS | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0378-1097 | ||||
Official Date: | 1 March 1991 | ||||
Dates: |
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Volume: | 78 | ||||
Number: | 2-3 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 121-126 | ||||
Publication Status: | Published |
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