UNSPECIFIED. (1991) CLONING OF NITROGENASE STRUCTURAL GENES FROM THE OBLIGATE METHANOTROPH METHYLOCOCCUS-CAPSULATUS (BATH). FEMS MICROBIOLOGY LETTERS, 78 (2-3). pp. 121-126. ISSN 0378-1097

Abstract

Southern hybridization techniques were used to examine the DNA homologies between the three nitrogenase structural genes nif H, nif D and nif K of Klebsiella pneumoniae and DNA from the obligate methane oxidizing bacterium Methylococcus capsulatus (Bath). The high degree of homology between methanotroph DNA sequences and the Klebsiella nif H and nif D genes was used to isolate and clone the corresponding Methylococcus nif H and nif D genes. Subsequent restriction analysis revealed that all three nif structural genes were contiguous in the Methylococcus genome in the order nif H, nif D and nif K, as found for the majority of other diazotrophs.

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