UNSPECIFIED (1991) SEQUENCE OF THE MAJOR NUCLEOCAPSID PROTEIN GENE OF PNEUMONIA VIRUS OF MICE - SEQUENCE COMPARISONS SUGGEST STRUCTURAL HOMOLOGY BETWEEN NUCLEOCAPSID PROTEINS OF PNEUMOVIRUSES, PARAMYXOVIRUSES, RHABDOVIRUSES AND FILOVIRUSES. JOURNAL OF GENERAL VIROLOGY, 72 (Part 3). pp. 677-685. ISSN 0022-1317

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Abstract

The complete nucleotide sequence of gene 3 of pneumonia virus of mice has been determined, and the 5' end of the mRNA mapped using a modification of the polymerase chain reaction technique. The gene contains a single open reading frame, beginning with a 5'-proximal AUG initiation codon, encoding a polypeptide with a predicted M(r) of 43141. Expression of the gene 3 protein in Escherichia coli and in vitro showed that it reacted with virus-specific antiserum and comigrated with the major nucleocapsid (N) polypeptide. The predicted amino acid sequence has extensive identity with that of the N protein of human respiratory syncytial virus. Comparisons with the amino acid sequences of N proteins of other paramyxoviruses, vesicular stomatitis virus and Ebola virus suggest that these proteins may have retained much of the same structure. These regions of conserved structure would most likely have the common functions of RNA binding and protein/protein interactions in the virus nucleocapsid.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title:	JOURNAL OF GENERAL VIROLOGY
Publisher:	SOC GENERAL MICROBIOLOGY
ISSN:	0022-1317
Date:	March 1991
Volume:	72
Number:	Part 3
Number of Pages:	9
Page Range:	pp. 677-685
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/22816

Data sourced from Thomson Reuters' Web of Knowledge

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