INTERFERON-ALPHA RECEPTOR EXPRESSION AND REGULATION IN CHRONIC HEPATITIS-B VIRUS-INFECTION
UNSPECIFIED. (1991) INTERFERON-ALPHA RECEPTOR EXPRESSION AND REGULATION IN CHRONIC HEPATITIS-B VIRUS-INFECTION. HEPATOLOGY, 13 (2). pp. 332-338. ISSN 0270-9139Full text not available from this repository.
Interferon-alpha elicits antiviral and immunoregulatory activities by binding to specific receptors on the cell surface. In this study, binding characteristics of interferon-alpha to peripheral blood mononuclear cells in patients with chronic hepatitis B virus infection were studied using radioiodinated recombinant interferon-alpha-2b to determine interferon-alpha receptor numbers and dissociation constants. A single class of interferon-alpha receptor was demonstrated on peripheral blood mononuclear cells and mononuclear subsets. Peripheral blood mononuclear cells from patients with chronic hepatitis B virus infection (n = 20) and controls (n = 16) expressed a similar number of interferon-alpha receptors (484 +/- 175 vs. 511 +/- 168 sites/cell respectively, p = NS) with a similar dissociation constant (dissociation constant almost-equal-to 0.2 to 0.7 nmol/L). Expression of interferon-alpha receptors was similar in monocyte-enriched and lymphocyte-enriched fractions in both groups. Similar changes were observed in patients receiving alpha-interferon therapy. there was no correlation between interferon-alpha receptors expression and serum transaminase, serum HBsAg, serum HBV DNA, liver histological findings or the response to interferon-alpha therapy.
After incubation of lymphocytes in vitro with interferon-alpha-2b (10 to 1,000 U/ml), interferon-alpha receptors number dropped by 42% to 80%, but this was associated with an increase in binding affinity (dissociation constant almost-equal-to 0.05 to 0.15 nmol/L in both patients and controls. There was significant delay in the initial phase of receptor recovery in the patients with chronic hepatitis B virus infection compared with normal controls (days 1 and 2, p < 0.05).
These data indicate that interferon-alpha receptors are expressed and regulated normally in chronic hepatitis B virus infection and that the variable response to interferon-alpha therapy is not due to a variation in interferon-alpha receptor. The increase in binding affinity on interferon-alpha therapy may be one factor explaining why long-term interferon-alpha therapy is effective despite a decrease in receptor number.
|Item Type:||Journal Article|
|Subjects:||R Medicine > RC Internal medicine|
|Journal or Publication Title:||HEPATOLOGY|
|Publisher:||W B SAUNDERS CO|
|Official Date:||February 1991|
|Number of Pages:||7|
|Page Range:||pp. 332-338|
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