UNSPECIFIED. (1991) MOLECULAR ANALYSIS OF THE METHANE MONOOXYGENASE (MMO) GENE-CLUSTER OF METHYLOSINUS-TRICHOSPORIUM OB3B. MOLECULAR MICROBIOLOGY, 5 (2). pp. 335-342. ISSN 0950-382X

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Abstract

The oxidation of methane to methanol in methanotrophic bacteria is catalysed by the enzyme methane monooxygenase (MMO). This multicomponent enzyme catalyses a range of oxidations including that of aliphatic and aromatic compounds and therefore has potential for commercial exploitation. This study details the molecular characterization of the soluble MMO (sMMO) genes from the Type II methanotroph Methylosinus trichosporium OB3b. The structural genes encoding the alpha, beta and gamma subunits of sMMO protein A and the structural gene encoding component B have been isolated and sequenced. These genes have been expressed and their products identified using an in vitro system. A comparative analysis of sMMO predicted sequences of M. trichosporium OB3b and the toxonomically related M. capsulatus (Bath) is also presented.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology
Journal or Publication Title:	MOLECULAR MICROBIOLOGY
Publisher:	BLACKWELL SCIENCE LTD
ISSN:	0950-382X
Date:	February 1991
Volume:	5
Number:	2
Number of Pages:	8
Page Range:	pp. 335-342
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/22833

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