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Dissecting the genetic regulation of texture traits in tomato fruit
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Walley, Peter Glen (2007) Dissecting the genetic regulation of texture traits in tomato fruit. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b2280258~S9
Abstract
The aim of the work presented in this thesis was to assess the genetic variation present within the wild tomato species Solanum pennellii that can be adapted to improve the texture of the domesticated tomato species. Using a population of S. pennellii introgression lines, 23 significant QTL supporting intervals were identified. Nine of the QTL were significant in two growing seasons. Three QTL were identified for pericarp firmness. Lines containing the firmness QTL F-Sp 2.1 were used to create BC1 populations. Sensory analyses were used to correlate the instrumental texture measurements to those perceived during mastication. Repeated texture measurements were conducted on lines representing similar chromosomal regions from another wild tomato species Solanum habrochaites introgression line population. To better understand the genetic basis of one of the firmness QTL identified, the Syngenta tomato Affymetrix GeneChip was used to quantify the differential expression of S. pennellii genes within the QTL-introgression line through development in comparison to the recurrent parent S. lycopersicum L. cv M82. The microarray analyses were extended to the ripening mutants Cnr, nor and rin. Differential gene expression between the ripening mutants and the wild type Ailsa Craig were compared through development. Candidate genes for the firmness QTL and fruit development were nominated.
| Item Type: | Thesis or Dissertation (PhD) |
|---|---|
| Subjects: | S Agriculture > SB Plant culture Q Science > QH Natural history > QH426 Genetics |
| Library of Congress Subject Headings (LCSH): | Tomatoes -- Genetics -- Research, Tomatoes -- Varieties, Food texture, DNA microarrays |
| Date: | September 2007 |
| Institution: | University of Warwick |
| Theses Department: | Warwick HRI |
| Thesis Type: | PhD |
| Publication Status: | Unpublished |
| Supervisor(s)/Advisor: | Seymour, G. B. (Graham B.) |
| Sponsors: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) ; Syngenta Seeds Ltd. (SSL) |
| Extent: | xxiii, 341 leaves : ill., charts. + 1 CD-ROM |
| Language: | eng |
| URI: | http://wrap.warwick.ac.uk/id/eprint/2291 |
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