The Library
Origins of the amphiploid species Brassica napus L. investigated by chloroplast and nuclear molecular markers
Tools
Allender, Charlotte J. and King , Graham J. (2010) Origins of the amphiploid species Brassica napus L. investigated by chloroplast and nuclear molecular markers. BMC Plant Biology, Vol.10 (Article 54). doi:10.1186/1471-2229-10-54 ISSN 1471-2229.
PDF
WRAP_Allender_0380313-hr-210410-cja_bmcplbiol_2010_revfinal_checked_2.pdf - Requires a PDF viewer. Download (244Kb) |
Official URL: http://dx.doi.org/10.1186/1471-2229-10-54
Abstract
Background: The amphiploid species Brassica napus (oilseed rape, Canola) is a globally important oil crop yielding food, biofuels and industrial compounds such as lubricants and surfactants. Identification of the likely ancestors of each of the two genomes (designated A and C) found in B. napus would facilitate incorporation of novel alleles from the wider Brassica genepool in oilseed rape crop genetic improvement programmes. Knowledge of the closest extant relatives of the genotypes involved in the initial formation of B. napus would also allow further investigation of the genetic factors required for the formation of a stable amphiploid and permit the more efficient creation of fully fertile re-synthesised B. napus. We have used a combination of chloroplast and nuclear genetic markers to investigate the closest extant relatives of the original maternal progenitors of B. napus. This was based on a comprehensive sampling of the relevant genepools, including 83 accessions of A genome B. rapa L. (both wild and cultivated types), 94 accessions of B. napus and 181 accessions of C genome wild and cultivated B. oleracea L. and related species.
Results: Three chloroplast haplotypes occurred in B. napus. The most prevalent haplotype (found in 79% of accessions) was not present within the C genome accessions but was found at low frequencies in B. rapa. Chloroplast haplotypes characteristic of B. napus were found in a small number of wild and weedy B. rapa populations, and also in two accessions of cultivated B. rapa 'brocoletto'. Whilst introgression of the B. napus chloroplast type in the wild and weedy B. rapa populations has been proposed by other studies, the presence of this haplotype within the two brocoletto accessions is unexplained.
Conclusions: The distribution of chloroplast haplotypes eliminates any of the C genome species as being the maternal ancestor of the majority of the B. napus accessions. The presence of multiple chloroplast haplotypes in B. napus and B. rapa accessions was not correlated with nuclear genetic diversity as determined by AFLPs, indicating that such accessions do not represent recent hybrids. Whilst some chloroplast diversity observed within B. napus can be explained by introgression from inter-specific crosses made during crop improvement programmes, there is evidence that the original hybridisation event resulting in to B. napus occurred on more than one occasion, and involved different maternal genotypes.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QK Botany Q Science > QH Natural history > QH426 Genetics |
||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) | ||||
Library of Congress Subject Headings (LCSH): | Rape (Plant) -- Genetics, Chloroplast DNA -- Research, Genetic markers -- Research, Hybridization -- Molecular aspects, Plant hybridization | ||||
Journal or Publication Title: | BMC Plant Biology | ||||
Publisher: | BioMed Central Ltd. | ||||
ISSN: | 1471-2229 | ||||
Official Date: | 29 March 2010 | ||||
Dates: |
|
||||
Volume: | Vol.10 | ||||
Number: | Article 54 | ||||
DOI: | 10.1186/1471-2229-10-54 | ||||
Status: | Peer Reviewed | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Funder: | Natural Environment Research Council (Great Britain) (NERC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA), University of Warwick |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year