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In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots

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Gattolin, Stefano, Sorieul, Mathias, Hunter, P. R. (Paul R.), Khonsari, Roman H. and Frigerio, Lorenzo. (2009) In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots. BMC Plant Biology, Vol.9 . Article 133. ISSN 1471-2229

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Official URL: http://dx.doi.org/10.1186/1471-2229-9-133

Abstract

Background: Tonoplast intrinsic proteins (TIPs) are widely used as markers for vacuolar compartments in higher plants. Ten TIP isoforms are encoded by the Arabidopsis genome. For several isoforms, the tissue and cell specific pattern of expression are not known. Results: We generated fluorescent protein fusions to the genomic sequences of all members of the Arabidopsis TIP family whose expression is predicted to occur in root tissues (TIP1;1 and 1;2; TIP2;1, 2;2 and 2;3; TIP4;1) and expressed these fusions, both individually and in selected pairwise combinations, in transgenic Arabidopsis. Analysis by confocal microscopy revealed that TIP distribution varied between different cell layers within the root axis, with extensive co-expression of some TIPs and more restricted expression patterns for other isoforms. TIP isoforms whose expression overlapped appeared to localise to the tonoplast of the central vacuole, vacuolar bulbs and smaller, uncharacterised structures. Conclusion: We have produced a comprehensive atlas of TIP expression in Arabidopsis roots, which reveals novel expression patterns for not previously studied TIPs.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Arabidopsis thaliana -- Genetics, Tonoplasts -- Research, Plant proteins, Roots (Botany) -- Physiology, Confocal microscopy
Journal or Publication Title:	BMC Plant Biology
Publisher:	BioMed Central Ltd.
ISSN:	1471-2229
Date:	18 November 2009
Volume:	Vol.9
Page Range:	Article 133
Identification Number:	10.1186/1471-2229-9-133
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Leverhulme Trust (LT), European Union (EU)
Grant number:	LSH-2002-1.2.5-2 (EU), F/00215/AP (LT)
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URI:	http://wrap.warwick.ac.uk/id/eprint/2337