Naylor, Richard William and Jones, E. A. (2009) Notch activates Wnt-4 signalling to control medio-lateral patterning of the pronephros. Development , Vol.136 (No.21). pp. 3585-3595. doi:10.1242/dev.042606

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Abstract

Previous studies have highlighted a role for the Notch signalling pathway during pronephrogenesis in the amphibian Xenopus laevis, and in nephron development in the mammalian metanephros, yet a mechanism for this function remains elusive. Here, we further the understanding of how Notch signalling patterns the early X. laevis pronephros anlagen, a function that might be conserved in mammalian nephron segmentation. Our results indicate that early phase pronephric Notch signalling patterns the medio-lateral axis of the dorso-anterior pronephros anlagen, permitting the glomus and tubules to develop in isolation. We show that this novel function acts through the Notch effector gene hrt1 by upregulating expression of wnt4. Wnt-4 then patterns the proximal pronephric anlagen to establish the specific compartments that span the medio-lateral axis. We also identified pronephric expression of lunatic fringe and radical fringe that is temporally and spatially appropriate for a role in regulating Notch signalling in the dorso-anterior region of the pronephros anlagen. On the basis of these results, along with data from previous publications, we propose a mechanism by which the Notch signalling pathway regulates a Wnt-4 function that patterns the proximal pronephric anlagen.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH426 Genetics
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Notch genes -- Research, Xenopus laevis, Gene expression -- Research, Cellular signal transduction
Journal or Publication Title:	Development
Publisher:	The Company of Biologists Ltd.
ISSN:	0950-1991
Official Date:	1 November 2009
Dates:	Date Event 1 November 2009 Published
Volume:	Vol.136
Number:	No.21
Page Range:	pp. 3585-3595
DOI:	10.1242/dev.042606
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
Grant number:	G1988 (BBSRC), G12713 (BBSRC)

Data sourced from Thomson Reuters' Web of Knowledge

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