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Release of ATP in the ventral medulla during hypoxia in rats: role in hypoxic ventilatory response

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Gourine, Alexander V., Llaudet, Enrique, Dale, Nicholas and Spyer, K. Michael. (2005) Release of ATP in the ventral medulla during hypoxia in rats: role in hypoxic ventilatory response. Journal of Neuroscience, Vol.25 (No.5). pp. 1211-1218. ISSN 0270-6474

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Official URL: http://dx.doi.org/10.1523/JNEUROSCI.3763-04.2005

Abstract

P2X2 receptor subunits of the ATP-gated ion channels are expressed by physiologically identified respiratory neurons in the ventral respiratory column, implicating ATP in the control of respiratory activity. We now show that, during hypoxia, release of ATP in the ventrolateral medulla (VLM) plays an important role in the hypoxic ventilatory response in rats. By measuring ATP release in real time at the ventral surface of the medulla with novel amperometric biosensors, we found that hypoxia (10% O2; 5 min) induced a marked increase in the concentration of ATP (~3 µM). This ATP release occurred after the initiation of enhanced respiratory activity but coincided with the later hypoxia-induced slowing of the respiratory rhythm. ATP was also released at the ventral surface of the medulla during hypoxia in peripherally chemodenervated animals (vagi, aortic, and carotid sinus nerve sectioned). By using horizontal slices of the rat medulla, we found that, during hypoxia, ATP is produced throughout the VLM in the locations corresponding to the ventral respiratory column. Blockade of ATP receptors in the VLM (microinjection of P2 receptor antagonist pyridoxal-5'-phosphate-6-azophenyl-2',4'-disulphonic acid; 100 µM) augmented the hypoxia-induced secondary slowing of the respiratory rhythm. Our findings suggest that ATP released within the ventral respiratory column is involved in maintenance of the respiratory activity in conditions when hypoxia-induced slowing of respiration occurs. These data illustrate a new functional role for ATP-mediated purinergic signaling in the medullary mechanisms controlling respiratory activity.

Item Type:

Journal Article

Subjects:

R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Q Science > QL Zoology

Divisions:

Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)

Library of Congress Subject Headings (LCSH):

Adenosine triphosphate, Medulla oblongata, Cerebral anoxia, Biosensors, Respiration -- Regulation, Rats -- Physiology

Journal or Publication Title:

Journal of Neuroscience

Publisher:

Society for Neuroscience

ISSN:

0270-6474

Official Date:

2 February 2005

Dates:

Date	Event
2 February 2005	Published

Volume:

Vol.25

Number:

No.5

Page Range:

pp. 1211-1218

Identification Number:

10.1523/JNEUROSCI.3763-04.2005

Status:

Peer Reviewed

Access rights to Published version:

Open Access

Funder:

Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Wellcome Trust (London, England)

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