Kloosterman, Bjorn, De Koeyer, David, Griffiths, Rebecca, Flinn , Barry, Steuernagel, Burkhard, Scholz, Uwe, Sonnewald, Sophia, Sonnewald, Uwe, Bryan, Glenn J., Prat, Salome, Bánfalvi, Zsófia, Hammond, John P., Geigenberger, Peter, Nielsen, Kåre L., Visser, Richard G. F. and Bachem, Christian W. B. (2008) Genes driving potato tuber initiation and growth : identification based on transcriptional changes using the POCI array. Functional & Integrative Genomics, Vol.8 (No.4). pp. 329-340. doi:10.1007/s10142-008-0083-x ISSN 1438-793X.

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Abstract

The increasing amount of available expressed gene
sequence data makes whole-transcriptome analysis of certain
crop species possible. Potato currently has the second largest number of publicly available expressed sequence tag (EST) sequences among the Solanaceae. Most of these ESTs, plus other proprietary sequences, were combined and used togenerate a unigene assembly. The set of 246,182 sequences
produced 46,345 unigenes, which were used to design a 44K
60-mer oligo array (Potato Oligo Chip Initiative: POCI). In
this study, we attempt to identify genes controlling and driving the process of tuber initiation and growth by implementing large-scale transcriptional changes using the newly developed POCI array. Major gene expression profiles could be identified exhibiting differential expression at key developmental stages. These profiles were associated with functional roles in cell division and growth. A subset of genes involved in the regulation of the cell cycle, based on their Gene Ontology classification, exhibit a clear transient upregulation at tuber onset indicating increased cell division during these stages. The POCI array allows the study of potato gene expression on a much broader level than previously possible and will greatly enhance analysis of transcriptional control mechanisms in a wide range of potato research areas. POCI sequence and annotation data are publicly available through the POCI database (http://pgrc.ipk-gatersleben.de/poci).

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Potatoes -- Genetics, Tubers
Journal or Publication Title:	Functional & Integrative Genomics
Publisher:	Springer
ISSN:	1438-793X
Official Date:	27 May 2008
Dates:	Date Event 27 May 2008 Published
Volume:	Vol.8
Number:	No.4
Page Range:	pp. 329-340
DOI:	10.1007/s10142-008-0083-x
Status:	Peer Reviewed
Access rights to Published version:	Open Access (Creative Commons)
Funder:	Genome Canada, Atlantic Innovation Fund of the Atlantic Canada Opportunities Agency (AIF), New Brunswick Department of Agriculture, Fisheries and Aquaculture (AFA), Scotland. Rural and Environment Research and Analysis Directorate (RERAD), European Union (EU), Centre of BioSystems Genomics (CBSG)
Grant number:	1004889-1 (AIF), AFA03/04-096 (AFA), PL 016214-2 (EU-SOL)

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